RPN7 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Detection of RPN7 (49 kDa) in the crude extract of S. cerevisiae by Western blotting using RPN7 polyclonal antibody (Cat # PAB15595).
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant RPN7.
Immunogen
Recombinant protein corresponding to Saccharomyces cerevisiae RPN7.
Host
Rabbit
Reactivity
Yeast
Specificity
This antibody react with S. cerevisiae RPN7.
Form
Liquid
Recommend Usage
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM Tris-HCl, 100 mM NaCl, pH 7.4 (0.05% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Detection of RPN7 (49 kDa) in the crude extract of S. cerevisiae by Western blotting using RPN7 polyclonal antibody (Cat # PAB15595).Immunoprecipitation
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Gene Info — RPN7
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Publication Reference
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The assembly pathway of the 19S regulatory particle of the yeast 26S proteasome.
Isono E, Nishihara K, Saeki Y, Yashiroda H, Kamata N, Ge L, Ueda T, Kikuchi Y, Tanaka K, Nakano A, Toh-e A.
Molecular Biology of the Cell 2007 Feb; 18(2):569.
Application：WB, Yeast, W303-1, YEK100 cells.
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Rpn7 Is required for the structural integrity of the 26 S proteasome of Saccharomyces cerevisiae.
Isono E, Saeki Y, Yokosawa H, Toh-e A.
The Journal of Biological Chemistry 2004 Jun; 279(26):27168.
Application：WB, Yeast, Yeast cells.
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The ubiquitin system.
Hershko A, Ciechanover A.
Annual Review of Biochemistry 1998 Jul; 67:425.
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The assembly pathway of the 19S regulatory particle of the yeast 26S proteasome.
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