RPN5 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Detection of RPN5 (52 kDa) in the crude extract of S. cerevisiae by Western blotting using RPN5 polyclonal antibody (Cat # PAB15594).
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant RPN5.
Immunogen
Recombinant protein corresponding to Saccharomyces cerevisiae RPN5.
Host
Rabbit
Reactivity
Yeast
Specificity
This antibody react with S. cerevisiae RPN5.
Form
Liquid
Recommend Usage
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM Tris-HCl, 100 mM NaCl, pH 7.4 (0.05% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Detection of RPN5 (52 kDa) in the crude extract of S. cerevisiae by Western blotting using RPN5 polyclonal antibody (Cat # PAB15594).Immunoprecipitation
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Gene Info — RPN5
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Publication Reference
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Endoplasmic Reticulum-associated Degradation of Pca1p, a Polytopic Protein, via Interaction with the Proteasome at the Membrane.
Smith N, Adle DJ, Zhao M, Qin X, Kim H and Lee J.
The Journal of Biological Chemistry 2016 Jul; 291(29):15082.
Application：WB-Ce, Yeast, S. cerevisiae .
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The assembly pathway of the 19S regulatory particle of the yeast 26S proteasome.
Isono E, Nishihara K, Saeki Y, Yashiroda H, Kamata N, Ge L, Ueda T, Kikuchi Y, Tanaka K, Nakano A, Toh-e A.
Molecular Biology of the Cell 2007 Feb; 18(2):569.
Application：WB, Yeast, W303-1, YEK100 cells.
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Unified nomenclature for subunits of the Saccharomyces cerevisiae proteasome regulatory particle.
Finley D, Tanaka K, Mann C, Feldmann H, Hochstrasser M, Vierstra R, Johnston S, Hampton R, Haber J, Mccusker J, Silver P, Frontali L, Thorsness P, Varshavsky A, Byers B, Madura K, Reed SI, Wolf D, Jentsch S, Sommer T, Baumeister W, Goldberg A, Fried V, Rubin DM, Toh-e A, et al..
Trends in Biochemical Sciences 1998 Jul; 23(7):244.
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The ubiquitin system.
Hershko A, Ciechanover A.
Annual Review of Biochemistry 1998 Jul; 67:425.
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Endoplasmic Reticulum-associated Degradation of Pca1p, a Polytopic Protein, via Interaction with the Proteasome at the Membrane.
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