RPN3 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Detection of RPN3 (60 kDa) in the crude extract of S. cerevisiae by Western blotting using RPN3 polyclonal antibody (Cat # PAB15593).
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant RPN3.
Immunogen
Recombinant protein corresponding to Saccharomyces cerevisiae RPN3.
Host
Rabbit
Reactivity
Yeast
Specificity
This antibody react with S. cerevisiae RPN3.
Form
Liquid
Recommend Usage
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In antiserum (0.05% sodium azide)
Storage Instruction
Store at 4°C.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Detection of RPN3 (60 kDa) in the crude extract of S. cerevisiae by Western blotting using RPN3 polyclonal antibody (Cat # PAB15593).Immunoprecipitation
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Gene Info — RPN3
Entrez GeneID
856742Gene Name
RPN3
Gene Alias
SUN2
Gene Description
Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control
Gene Ontology
HyperlinkGene Summary
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Other Designations
Rpn3p
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Publication Reference
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Rpn7 Is required for the structural integrity of the 26 S proteasome of Saccharomyces cerevisiae.
Isono E, Saeki Y, Yokosawa H, Toh-e A.
The Journal of Biological Chemistry 2004 Jun; 279(26):27168.
Application：WB, Yeast, Yeast cells.
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Unified nomenclature for subunits of the Saccharomyces cerevisiae proteasome regulatory particle.
Finley D, Tanaka K, Mann C, Feldmann H, Hochstrasser M, Vierstra R, Johnston S, Hampton R, Haber J, Mccusker J, Silver P, Frontali L, Thorsness P, Varshavsky A, Byers B, Madura K, Reed SI, Wolf D, Jentsch S, Sommer T, Baumeister W, Goldberg A, Fried V, Rubin DM, Toh-e A, et al..
Trends in Biochemical Sciences 1998 Jul; 23(7):244.
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The ubiquitin system.
Hershko A, Ciechanover A.
Annual Review of Biochemistry 1998 Jul; 67:425.
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Rpn7 Is required for the structural integrity of the 26 S proteasome of Saccharomyces cerevisiae.
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