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Last updated: 2017/8/13
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Dnmt1 polyclonal antibody

  • Catalog # : PAB15590
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against partial recombinant Dnmt1.
  • Immunogen:
  • Recombinant protein corresponding to amino acids 1-248 mouse Dnmt1.
  • Host:
  • Rabbit
  • Reactivity:
  • Frog, Human, Mouse
  • Specificity:
  • This antibody reacts with Dnmt1.
  • Form:
  • Liquid
  • Recommend Usage:
  • Western Blot (0.2-1 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In antiserum (0.05% sodium azide)
  • Storage Instruction:
  • Store at -20°C. For long term storage store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Association of Np95 with Dnmt1 and Pcna in HeLa cell nuclear extracts.
    Human NP95 was tagged by biotin-binding domain and stably expressed in HeLa cells together with the E. coli BirA biotin ligase gene.
    Biotylated Np95 was captured by streptoavidin beads.
    The captured proteins were eluted from the beads and analyzed by western bloting using antibodies against Dnmt1 and Pcna.
    ( The data were kindly provided by Dr. M. Muto of Riken Research Center for Allergy and Immunology ).
  • Western Blot
  • Western Blot
  • Catalytic activity of NP95 associated Dnmt1 in HeLa cells.
    The presence of Dnmt1 in the NP95 complexes was confirmed by western blot and the DNA methyltransferase activity of the extracts was measured (The data were kindly provided by Dr. M. Muto of Riken Research Center for Allergy and Immunology).
  • Immunofluorescence
  • Immunofluorescence
  • Subnuclear localization of Dnmt1 (green), Np95 (red) and Pcna (blue) in mouse embryonic stem cells (E14) during cell-cycle progression (bottom).
    Merged images for Np95 and Dnmt1 are shown. Profile of DNA content in exponentially growing ECSs is shown (top).
    (The data were kindly provided by Dr. M. Muto of Riken Research Center for Allergy and Immunology).
  • Immunoprecipitation
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunoprecipitation
  • Gene Information
  • Gene Name:
  • Dnmt1
  • Gene Alias:
  • Cxxc9,Dnmt,Dnmt1o,MTase,Met-1,Met1,MommeD2
  • Gene Description:
  • DNA methyltransferase (cytosine-5) 1
  • Other Designations:
  • -
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