Dnmt1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of Dnmt1 KO cell extract. (1) Mouse ES cell line J1 (Dnmt1 +/+), and (2) Mouse ES cell line 36 (Dnmt1 -/-).
Western Blot
Western Blot analysis of (1) Immunogen, GST-Dnmt3 (1-248) fusion protein, and (2) Whole cell extract of NIH3T3 cells (20 ug). The Dnmt1 polyclonal antibody was used at 1 ug/mL and 2nd antibody, goat anti-rabbit IgG antibody conjugated with HRP was used at 1/10000 dilution.
Immunofluorescence
Immunofluorescent staining of embryonic stem cells (E14) during cell-cycle progression were reacted with Dnmt1 polyclonal antibody as primary antibody at 1/5000 and with goat anti-rabbit IgG conjugated with Alexa Fluora 555 at 1/2000. Nuclei were stained with DAPI.
Immunoprecipitation
Immunoprecipitation of mouse MEL cells. The Dnmt1 polyclonal antibody attached to protein A conjugated magnetic beads was used. Non-immunized IgG was used as negative control.
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant Dnmt1.
Immunogen
Recombinant protein corresponding to amino acids 1-248 mouse Dnmt1.
Host
Rabbit
Reactivity
Human, Mouse
Specificity
This antibody reacts with Dnmt1.
Form
Liquid
Purity
Affinity-purified with the recombinant Dnmt1.
Recommend Usage
Immunoprecipitation (Especially good for native form of Dnmt1 and useful for analysis of the protein complex containing Dnmt1. One ug of the antibody can precipitates 0.5~1.0 ug of Dnmt1.)
Indirect immuno-fluorescence staining (Excellent. Low background image will be obtained by using the antibody at 5000 fold dilution)
Western Blot (0.2-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol).
Storage Instruction
Store at -20°C. Do not freeze.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of Dnmt1 KO cell extract. (1) Mouse ES cell line J1 (Dnmt1 +/+), and (2) Mouse ES cell line 36 (Dnmt1 -/-).Western Blot
Western Blot analysis of (1) Immunogen, GST-Dnmt3 (1-248) fusion protein, and (2) Whole cell extract of NIH3T3 cells (20 ug). The Dnmt1 polyclonal antibody was used at 1 ug/mL and 2nd antibody, goat anti-rabbit IgG antibody conjugated with HRP was used at 1/10000 dilution.Immunofluorescence
Immunofluorescent staining of embryonic stem cells (E14) during cell-cycle progression were reacted with Dnmt1 polyclonal antibody as primary antibody at 1/5000 and with goat anti-rabbit IgG conjugated with Alexa Fluora 555 at 1/2000. Nuclei were stained with DAPI.Immunoprecipitation
Immunoprecipitation of mouse MEL cells. The Dnmt1 polyclonal antibody attached to protein A conjugated magnetic beads was used. Non-immunized IgG was used as negative control. -
Gene Info — Dnmt1
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Publication Reference
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Critical Role of the UBL Domain in Stimulating the E3 Ubiquitin Ligase Activity of UHRF1 toward Chromatin.
Foster BM, Stolz P, Mulholland CB, Montoya A, Kramer H, Bultmann S, Bartke T.
Molecular Cell 2018 Nov; 72(4):739.
Application：IF, Mouse, Mouse embryonic stem cells (mESCs).
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Epigenetics of eu- and heterochromatin in inverted and conventional nuclei from mouse retina.
Eberhart A, Feodorova Y, Song C, Wanner G, Kiseleva E, Furukawa T, Kimura H, Schotta G, Leonhardt H, Joffe B, Solovei I.
Chromosome Research 2013 Aug; 21(5):535.
Application：IS, Mouse, Retina.
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The SRA protein Np95 mediates epigenetic inheritance by recruiting Dnmt1 to methylated DNA.
Sharif J, Muto M, Takebayashi S, Suetake I, Iwamatsu A, Endo TA, Shinga J, Mizutani-Koseki Y, Toyoda T, Okamura K, Tajima S, Mitsuya K, Okano M, Koseki H.
Nature 2007 Dec; 450(7171):908.
Application：IF, WB-Ce, Human, HeLa cells, Human embryonic stem cells .
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DNMT1 and DNMT3b cooperate to silence genes in human cancer cells.
Rhee I, Bachman KE, Park BH, Jair KW, Yen RW, Schuebel KE, Cui H, Feinberg AP, Lengauer C, Kinzler KW, Baylin SB, Vogelstein B.
Nature 2002 Apr; 416(6880):552.
Application：WB-Tr, Human, DKO cells.
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Methyltransferase recruitment and DNA hypermethylation of target promoters by an oncogenic transcription factor.
Di Croce L, Raker VA, Corsaro M, Fazi F, Fanelli M, Faretta M, Fuks F, Lo Coco F, Kouzarides T, Nervi C, Minucci S, Pelicci PG.
Science 2002 Feb; 295(5557):1079.
Application：WB-Ce, Human, NB4, PR9 cells.
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Critical Role of the UBL Domain in Stimulating the E3 Ubiquitin Ligase Activity of UHRF1 toward Chromatin.
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