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Last updated: 2021/6/13
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Dnmt1 polyclonal antibody 

  • Catalog # : PAB15590
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against partial recombinant Dnmt1.
  • Immunogen:
  • Recombinant protein corresponding to amino acids 1-248 mouse Dnmt1.
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Mouse
  • Specificity:
  • This antibody reacts with Dnmt1.
  • Form:
  • Liquid
  • Purity:
  • Affinity-purified with the recombinant Dnmt1.
  • Recommend Usage:
  • Immunoprecipitation (Especially good for native form of Dnmt1 and useful for analysis of the protein complex containing Dnmt1. One ug of the antibody can precipitates 0.5~1.0 ug of Dnmt1.)
    Indirect immuno-fluorescence staining (Excellent. Low background image will be obtained by using the antibody at 5000 fold dilution)
    Western Blot (0.2-1 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (50% glycerol).
  • Storage Instruction:
  • Store at -20°C. Do not freeze.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western Blot analysis of Dnmt1 KO cell extract. (1) Mouse ES cell line J1 (Dnmt1 +/+), and (2) Mouse ES cell line 36 (Dnmt1 -/-).
  • Western Blot
  • Western Blot
  • Western Blot analysis of (1) Immunogen, GST-Dnmt3 (1-248) fusion protein, and (2) Whole cell extract of NIH3T3 cells (20 ug). The Dnmt1 polyclonal antibody was used at 1 ug/mL and 2nd antibody, goat anti-rabbit IgG antibody conjugated with HRP was used at 1/10000 dilution.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent staining of embryonic stem cells (E14) during cell-cycle progression were reacted with Dnmt1 polyclonal antibody as primary antibody at 1/5000 and with goat anti-rabbit IgG conjugated with Alexa Fluora 555 at 1/2000. Nuclei were stained with DAPI.
  • Immunoprecipitation
  • Immunoprecipitation
  • Immunoprecipitation of mouse MEL cells. The Dnmt1 polyclonal antibody attached to protein A conjugated magnetic beads was used. Non-immunized IgG was used as negative control.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Gene Information
  • Gene Name:
  • Dnmt1
  • Gene Alias:
  • Cxxc9,Dnmt,Dnmt1o,MTase,Met-1,Met1,MommeD2
  • Gene Description:
  • DNA methyltransferase (cytosine-5) 1
  • Other Designations:
  • -
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