Sgo1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant Sgo1.
Immunogen
Recombinant protein corresponding to Schizosaccharomyces pombe Sgo1.
Host
Rabbit
Reactivity
S. pombe
Form
Liquid
Recommend Usage
Western Blot (1:1000)
Immunofluorescence (1:100)
The optimal working dilution should be determined by the end user.Storage Buffer
In antiserum (0.05% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Detection of Shugoshin 1 protein by Western blotting.
Sample : Extract of S. pombe MP111 cells.
Sgo1 polyclonal antibody (Cat # PAB15588) was diluted 1000 fold before use.Immunocytochemistry
Immunostaining of Shugoshin 1 in synchronized diploid fission yeast.
Sgo 1 protein was detected with Sgo1 polyclonal antibody (Cat # PAB15588) and Alexa-488-conjugated anti-rabbit antibody.Immunofluorescence
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Gene Info — sgo1
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Publication Reference
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Heterochromatin links to centromeric protection by recruiting shugoshin.
Yamagishi Y, Sakuno T, Shimura M, Watanabe Y.
Nature 2008 Sep; 455(7210):251.
Application:WB, S. pombe, Whole-cell extracts from Schizosaccharomyces pombe.
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Shugoshin protects cohesin complexes at centromeres.
Watanabe Y, Kitajima TS.
Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences 2005 Mar; 360(1455):515.
Application:IF, WB, Human, HeLa, Mammalian cells.
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The conserved kinetochore protein shugoshin protects centromeric cohesion during meiosis.
Kitajima TS, Kawashima SA, Watanabe Y.
Nature 2004 Feb; 427(6974):510.
Application:WB, Yeast, Yeast cells.
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Heterochromatin links to centromeric protection by recruiting shugoshin.
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