Identification of the Cut5 protein in the crude extract of S. pombe with Cut5 polyclonal antibody (Cat # PAB15586). Samples were prepared by alkali-lysis of the cells followed by TCA precipitation of proteins. Lane 1 : Wild-type cells. Lane 2: The cut5-5Flag gene replacing the wild-type cut5 gene. Lane 3 : The cut5-13myc gene replacing the wild type gene. Lane 4 : The cut-TAP gene replacing the wild-type gene.