Cut5 polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Identification of the Cut5 protein in the crude extract of S. pombe with Cut5 polyclonal antibody (Cat # PAB15586).
Samples were prepared by alkali-lysis of the cells followed by TCA precipitation of proteins.
Lane 1 : Wild-type cells.
Lane 2: The cut5-5Flag gene replacing the wild-type cut5 gene.
Lane 3 : The cut5-13myc gene replacing the wild type gene.
Lane 4 : The cut-TAP gene replacing the wild-type gene.
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant Cut5.
Immunogen
Recombinant GST fusion protein corresponding to N-terminus half Schizosaccharomyces pombe Cut5.
Host
Rabbit
Reactivity
S. pombe
Specificity
This antibody reacts with S. pombe Cut5.
Form
Liquid
Recommend Usage
Western Blot (1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In antiserum (0.05% sodium azide)
Storage Instruction
Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Identification of the Cut5 protein in the crude extract of S. pombe with Cut5 polyclonal antibody (Cat # PAB15586).
Samples were prepared by alkali-lysis of the cells followed by TCA precipitation of proteins.
Lane 1 : Wild-type cells.
Lane 2: The cut5-5Flag gene replacing the wild-type cut5 gene.
Lane 3 : The cut5-13myc gene replacing the wild type gene.
Lane 4 : The cut-TAP gene replacing the wild-type gene. -
Gene Info — cut5
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Publication Reference
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Damage and replication checkpoint control in fission yeast is ensured by interactions of Crb2, a protein with BRCT motif, with Cut5 and Chk1.
Saka Y, Esashi F, Matsusaka T, Mochida S, Yanagida M.
Genes & Development 1997 Dec; 11(24):3387.
Application：IP, WB-Ce, WB-Tr, Yeast, Yeast cells.
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Fission yeast cut5 links nuclear chromatin and M phase regulator in the replication checkpoint control.
Saka Y, Fantes P, Sutani T, McInerny C, Creanor J, Yanagida M.
The EMBO Journal 1994 Nov; 13(22):5319.
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Damage and replication checkpoint control in fission yeast is ensured by interactions of Crb2, a protein with BRCT motif, with Cut5 and Chk1.
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