CDC37 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Detection of CDC37 protein in the crude extract of S. cerevisiae by Western blotting using CDC37 polyclonal antibody (Cat # PAB15584).
Lane 1 : 1000 dilution.
Lane 2 : 5000 dilution.
CDC37 protein has a molecular weight of 58.4 kD, but appeared as a 68 kD band in SDS-PAGE.
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant CDC37.
Immunogen
Recombinant protein corresponding to Saccharomyces cerevisiae CDC37.
Host
Rabbit
Reactivity
Yeast
Specificity
This antibody reacts with S. cerevisiae CDC37.
Form
Liquid
Recommend Usage
Western Blot (1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In antiserum (0.05% sodium azide)
Storage Instruction
Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Detection of CDC37 protein in the crude extract of S. cerevisiae by Western blotting using CDC37 polyclonal antibody (Cat # PAB15584).
Lane 1 : 1000 dilution.
Lane 2 : 5000 dilution.
CDC37 protein has a molecular weight of 58.4 kD, but appeared as a 68 kD band in SDS-PAGE.Immunoprecipitation
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Gene Info — CDC37
Entrez GeneID
851746Gene Name
CDC37
Gene Alias
SMO1
Gene Description
Cdc37p
Gene Ontology
HyperlinkOther Designations
Essential Hsp90p co-chaperone; necessary for passage through the START phase of the cell cycle; stabilizes protein kinase nascent chains and participates along with Hsp90p in their folding
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Publication Reference
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Cdc37 is a molecular chaperone with specific functions in signal transduction.
Kimura Y, Rutherford SL, Miyata Y, Yahara I, Freeman BC, Yue L, Morimoto RI, Lindquist S.
Genes & Development 1997 Jul; 11(14):1775.
Application：WB-Ce, WB-Re, Yeast, Recombinant protein, Yeast cells.
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Mammalian p50Cdc37 is a protein kinase-targeting subunit of Hsp90 that binds and stabilizes Cdk4.
Stepanova L, Leng X, Parker SB, Harper JW.
Genes & Development 1996 Jun; 10(12):1491.
Application：IP, WB-Ce, WB-Re, WB-Tr, Human, Insect, Mouse, ML-1, NIH/3T3, Sf9 cells.
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The selection of S. cerevisiae mutants defective in the start event of cell division.
Reed SI.
Genetics 1980 Jul; 95(3):561.
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Cdc37 is a molecular chaperone with specific functions in signal transduction.
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