CBX3 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Identification of CBX3 in baby hamster kidney extract with CBX3 polyclonal antibody (Cat # PAB15582) by western blotting.
Immunofluorescence
Immunofluorescent staining of CBX3 in baby hamster kidney cells with CBX3 polyclonal antibody (Cat # PAB15582). Cells were fixed with para-formaldehyde. The second antibody was Alexa Fluor 594- conjugated goat anti-rabbit IgG antibody.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of CBX3.
Immunogen
A synthetic peptide corresponding to human CBX3.
Sequence
WHSCPEDEAQ
Host
Rabbit
Reactivity
Hamster, Human
Form
Liquid
Recommend Usage
Western Blot (1:2000-1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 120 mM sodium phosphate buffer, pH 7.4 (50% glycerol)
Storage Instruction
Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Tissue lysate)
Identification of CBX3 in baby hamster kidney extract with CBX3 polyclonal antibody (Cat # PAB15582) by western blotting.Immunofluorescence
Immunofluorescent staining of CBX3 in baby hamster kidney cells with CBX3 polyclonal antibody (Cat # PAB15582). Cells were fixed with para-formaldehyde. The second antibody was Alexa Fluor 594- conjugated goat anti-rabbit IgG antibody.Immunoprecipitation
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Gene Info — CBX3
Entrez GeneID
11335Gene Name
CBX3
Gene Alias
HECH, HP1-GAMMA, HP1Hs-gamma
Gene Description
chromobox homolog 3 (HP1 gamma homolog, Drosophila)
Omim ID
604477Gene Ontology
HyperlinkGene Summary
At the nuclear envelope, the nuclear lamina and heterochromatin are adjacent to the inner nuclear membrane. The protein encoded by this gene binds DNA and is a component of heterochromatin. This protein also can bind lamin B receptor, an integral membrane protein found in the inner nuclear membrane. The dual binding functions of the encoded protein may explain the association of heterochromatin with the inner nuclear membrane. Two transcript variants encoding the same protein but differing in the 5' UTR, have been found for this gene. [provided by RefSeq
Other Designations
HP1 gamma homolog|OTTHUMP00000024529|OTTHUMP00000122519|chromobox homolog 3|heterochromatin protein HP1 gamma|heterochromatin-like protein 1
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Interactome
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Publication Reference
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The Heterochromatin Protein 1 family.
Lomberk G, Wallrath L, Urrutia R.
Genome Biology 2006 Jul; 7(7):228.
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The assembly and maintenance of heterochromatin initiated by transgene repeats are independent of the RNA interference pathway in mammalian cells.
Wang F, Koyama N, Nishida H, Haraguchi T, Reith W, Tsukamoto T.
Molecular and Cellular Biology 2006 Jun; 26(11):4028.
Application：IF, ChIP, Mouse, Clone 2 cells.
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Interaction of the chromatin compaction-inducing domain (LR domain) of Ki-67 antigen with HP1 proteins.
Kametaka A, Takagi M, Hayakawa T, Haraguchi T, Hiraoka Y, Yoneda Y.
Genes to Cells 2002 Dec; 7(12):1231.
Application：IF, Human, HeLa cells.
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The Heterochromatin Protein 1 family.
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