RNQ1 polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Detection of RNQ1 protein in S. cerevisiae by Western blotting with RNQ1 polyclonal antibody (Cat # PAB15579).
Cells were harvested after 24 h of galactose induction.
Extracts were centrifuged and soluble (Lane 1) and pelleted (Lane 2) fractions were assayed by Western blotting using this antibody. RNQ1 protein was detected in pelleted fraction.
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Specifications
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of RNQ1.
Immunogen
A synthetic peptide corresponding to C-terminus of Saccharomyces cerevisiae RNQ1.
Sequence
SQQNNNGNQNRY
Host
Rabbit
Reactivity
Yeast
Specificity
This antibody reacts with S. cerevisiae RNQ1, not tested with other species.
Form
Liquid
Recommend Usage
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol).
Storage Instruction
Store at -20°C. Do not freeze.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Cell lysate)
Detection of RNQ1 protein in S. cerevisiae by Western blotting with RNQ1 polyclonal antibody (Cat # PAB15579).
Cells were harvested after 24 h of galactose induction.
Extracts were centrifuged and soluble (Lane 1) and pelleted (Lane 2) fractions were assayed by Western blotting using this antibody. RNQ1 protein was detected in pelleted fraction. -
Gene Info — RNQ1
Entrez GeneID
850329Protein Accession#
P25367Gene Name
RNQ1
Gene Alias
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Gene Description
Rnq1p
Gene Ontology
HyperlinkGene Summary
an infectious protein conformation that is generally an ordered protein aggregate
Other Designations
[PIN(+)] prion, an infectious protein conformation that is generally an ordered protein aggregate
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Publication Reference
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The role of pre-existing aggregates in Hsp104-dependent polyglutamine aggregate formation and epigenetic change of yeast prions.
Kimura Y, Koitabashi S, Kakizuka A, Fujita T.
Genes to Cells: Devoted to Molecular & Cellular Mechanisms 2004 Aug; 9(8):685.
Application：WB, Yeast , Yeast extracts.
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Rnq1: an epigenetic modifier of protein function in yeast.
Sondheimer N, Lindquist S.
Molecular Cell 2000 Jan; 5(1):163.
Application：WB, Yeast, Yeast cells.
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The role of pre-existing aggregates in Hsp104-dependent polyglutamine aggregate formation and epigenetic change of yeast prions.
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