Detection of SUP35 protein in crude extract of S. cerevisiae by Western blotting with SUP35 polyclonal antibody (Cat # PAB15578). Lane 1, 2 : 4000 dilution. Lane 3, 4 : 1000 dilution. Lane 1, 3 : endogenous expression. Lane 2, 4 : overexpression.
Translation termination factor eRF3; altered protein conformation creates the [PSI(+)] prion, a dominant cytoplasmically inherited protein aggregate that alters translational fidelity and creates a nonsense suppressor phenotype