Identification of RAD18 protein in crude extract of A-549 cells by western blotting with RAD18 polyclonal antibody (Cat # PAB15575). The lower thick band is native RAD18 and the upper thin band is mono-ubiquitinated RAD18 protein.
Indirect immunofluorescence staining of RAD18 protein in GM637 cells by RAD18 polyclonal antibody (Cat # PAB15575). RAD18 protein is stained as yellow dots in nuclei.
The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens. [provided by RefSeq
RAD18, S. cerevisiae, homolog,postreplication repair protein hRAD18p