Escherichia coli UmuD polyclonal antibody
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More Files
- More Functions
Western Blot (Recombinant protein)
Detection of UmuD protein in the extract of E. coli DE274 (LexA51, RecA730) by Western blotting using UmuD polyclonal antibody (Cat # PAB15572).
Lane 1 : without mitomycin C treatment.
Lane2 : treated with mitomycin C.
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant Escherichia coli UmuD.
Immunogen
Recombinant protein corresponding to Escherichia coli UmuD.
Host
Rabbit
Reactivity
Escherichia coli
Form
Liquid
Recommend Usage
Western Blot (1:3000)
The optimal working dilution should be determined by the end user.Storage Buffer
In antiserum (0.05% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Recombinant protein)
Detection of UmuD protein in the extract of E. coli DE274 (LexA51, RecA730) by Western blotting using UmuD polyclonal antibody (Cat # PAB15572).
Lane 1 : without mitomycin C treatment.
Lane2 : treated with mitomycin C. -
Gene Info — umuD
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Publication Reference
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RecA protein-dependent cleavage of UmuD protein and SOS mutagenesis.
Shinagawa H, Iwasaki H, Kato T, Nakata A.
PNAS 1988 Mar; 85(6):1806.
Application：IP, WB-Re, WB-Tr, Bacteria, Bacteria cells, Recombinant proteins.
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Structural analysis of the umu operon required for inducible mutagenesis in Escherichia coli.
Kitagawa Y, Akaboshi E, Shinagawa H, Horii T, Ogawa H, Kato T.
PNAS 1985 Jul; 82(13):4336.
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RecA protein-dependent cleavage of UmuD protein and SOS mutagenesis.
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