Escherichia coli RuvB polyclonal antibody
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More Files
- More Functions
Western Blot (Recombinant protein)
Detection of RuvB (37kD) protein by Western blotting using this antibody.
Lane1 : RuvB protein 5 ng.
Lane2 : E. coli AB1157 crude extract.
Lane3 : E. coli AB1157 LexA mutant crude extract.
Expression of RuvB is enhanced by LexA mutation.
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Specification
Product Description
Rabbit polyclonal antibody raised against full length recombinant Escherichia coli RuvB.
Immunogen
Recombinant protein corresponding to full length Escherichia coli RuvB.
Host
Rabbit
Reactivity
Escherichia coli
Form
Liquid
Recommend Usage
Western Blot (1:3000)
The optimal working dilution should be determined by the end user.Storage Buffer
In antiserum (0.05% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Recombinant protein)
Detection of RuvB (37kD) protein by Western blotting using this antibody.
Lane1 : RuvB protein 5 ng.
Lane2 : E. coli AB1157 crude extract.
Lane3 : E. coli AB1157 LexA mutant crude extract.
Expression of RuvB is enhanced by LexA mutation. -
Gene Info — ruvB
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Publication Reference
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Processing the holliday junction in homologous recombination.
Shinagawa H, Iwasaki H.
Trends in Biochemical Sciences 1996 Mar; 21(3):107.
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Escherichia coli RuvA and RuvB proteins specifically interact with Holliday junctions and promote branch migration.
Iwasaki H, Takahagi M, Nakata A, Shinagawa H.
Genes & Development 1992 Nov; 6(11):2214.
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Processing the holliday junction in homologous recombination.
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