SPHK1 polyclonal antibody
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More Files
- More Functions
Immunoprecipitation
Western blot and Immunoprecipitation of whole cell lysate from HeLa (5, 15 and 50 ug for WB ; 1 mg for IP, 20% of IP loaded) and 293T (50 ug) cells. Using SPHK1 polyclonal antibody (Cat # PAB15559).
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Specification
Product Description
Rabbit polyclonal antibody raised against SPHK1.
Immunogen
Amino acids 334-384 of human SPHK1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Recommend Usage
Immunoprecipitation (5-15 ug/mg lysate)
Western Blot (1:2000-1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris-citrate/phosphate, pH 7-8 (0.09% sodium azide)
Storage Instruction
Store at 4°C. Do not freeze.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunoprecipitation
Western blot and Immunoprecipitation of whole cell lysate from HeLa (5, 15 and 50 ug for WB ; 1 mg for IP, 20% of IP loaded) and 293T (50 ug) cells. Using SPHK1 polyclonal antibody (Cat # PAB15559). -
Gene Info — SPHK1
Entrez GeneID
8877Protein Accession#
NP_001136073.1Gene Name
SPHK1
Gene Alias
SPHK
Gene Description
sphingosine kinase 1
Omim ID
603730Gene Ontology
HyperlinkGene Summary
Sphingosine-1-phosphate (SPP) is a novel lipid messenger with both intracellular and extracellular functions. Intracellularly, it regulates proliferation and survival, and extracellularly, it is a ligand for EDG1 (MIM 601974). Various stimuli increase cellular levels of SPP by activation of sphingosine kinase (SPHK), the enzyme that catalyzes the phosphorylation of sphingosine. Competitive inhibitors of SPHK block formation of SPP and selectively inhibit cellular proliferation induced by a variety of factors, including platelet-derived growth factor (e.g., MIM 173430) and serum.[supplied by OMIM
Other Designations
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Interactome
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Pathway
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