Histone H3 (K4me3) polyclonal antibody
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More Files
- More Functions
ChIP
Histone H3 (K4me3) polyclonal antibody (Cat # PAB15454) was used in ChIP with wild type yeast chromatin (Lane 1) ora strain with a point mutation in the code for Lysine 4 (Lane 2).
Western Blot (Cell lysate)
Acid treated HeLa (10 ug) extract was treated with a 1 : 100 dilution of Histone H3 (K4me3) polyclonal antibody (Cat # PAB15454) prior to visualization.
Dot Blot (Peptide)
To confrm the specifcity of the antisera a dot blot system was used with amounts of peptides from 10-250 pmoles.
Unmodifed, monomethylated, dimethylated and trimethylated peptides surrounding the lysine 4 site on Histone H3 were spotted in lanes 1-4 respectively.
Lanes 5-8 contained peptides starting at residue 6, with the sequence, TARKSTGGKAPRKQLAT, encompassing the residue at lysine 9 on Histone H3 that is unmodifed, monomethylated, dimethylated and trimethylated respectively.
Lanes 9-12 contained peptides encompassing the residue at lysine 27 on Histone H3 that is unmodi-fed, monomethylated, dimethylated and trimethylated respectively.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of Histone H3 (K4me3).
Immunogen
A synthetic lysine4 trimethylated peptide of Histone H3 (K4me3).
Host
Rabbit
Reactivity
Yeast
Specificity
This antibody is expected to react with Histone H3 from yeast to mammals.
Form
Liquid
Recommend Usage
Western Blot (1:100-1:500)
ChIP (30-50 ul)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
ChIP
Histone H3 (K4me3) polyclonal antibody (Cat # PAB15454) was used in ChIP with wild type yeast chromatin (Lane 1) ora strain with a point mutation in the code for Lysine 4 (Lane 2).Western Blot (Cell lysate)
Acid treated HeLa (10 ug) extract was treated with a 1 : 100 dilution of Histone H3 (K4me3) polyclonal antibody (Cat # PAB15454) prior to visualization.Dot Blot (Peptide)
To confrm the specifcity of the antisera a dot blot system was used with amounts of peptides from 10-250 pmoles.
Unmodifed, monomethylated, dimethylated and trimethylated peptides surrounding the lysine 4 site on Histone H3 were spotted in lanes 1-4 respectively.
Lanes 5-8 contained peptides starting at residue 6, with the sequence, TARKSTGGKAPRKQLAT, encompassing the residue at lysine 9 on Histone H3 that is unmodifed, monomethylated, dimethylated and trimethylated respectively.
Lanes 9-12 contained peptides encompassing the residue at lysine 27 on Histone H3 that is unmodi-fed, monomethylated, dimethylated and trimethylated respectively.
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