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Last updated: 2016/12/4
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Histone H3 (K4me2) polyclonal antibody

  • Catalog # : PAB15453
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of Histone H3 (K4me2).
  • Immunogen:
  • Lysine4 dimethylated peptide of Histone H3.
  • Host:
  • Rabbit
  • Reactivity:
  • Yeast
  • Specificity:
  • This antibody is expected to react with Histone H3 from yeast to mammals.
  • Form:
  • Liquid
  • Recommend Usage:
  • Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.2 (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • ChIP
  • ChIP
  • Histone H3 (K4me2) polyclonal antibody (Cat # PAB15453) was used in ChIP with murine embryonic stem cells (Lane 1) or erythroleukemia cells (Lane 2).
    Top Panel : PCR primers specifc for beta-globulin were used to amplify a 210 base pair promoter following DNA isolation.
    Lower Panel : Input DNA control.
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Acid treated HeLa (10 ug) extract was treated with a 1 : 500 dilution of antibody Histone H3 (K4me2) polyclonal antibody (Cat # PAB15453) prior to visualization.
  • Dot Blot (Peptide)
  • Dot Blot (Peptide)
  • To confrm the specifcity of the antisera a dot blot system was used with amounts of peptides from 10-250 pmoles.
    Unmodifed, monomethylated, dimethylated and trimethylated peptides surrounding the Lysine 4 site on Histone H3 were spotted in lanes 1-4 respectively.
    Lanes 5-8 contained peptides starting at residue 6, with the sequence, TARKSTGGKAPRKQLAT, encompassing the residue lysine 9 on Histone H3 that is unmodifed, monomethylated, dimethylated and trimethylated respectively.
    Lanes 9-12 contained peptides encompassing the residue lysine 27 on Histone H3 that is unmodifed, monomethylated, dimethylated and trimethylated respectively.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
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