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Histone H3 (K4me1) polyclonal antibody

  • Catalog # : PAB15452
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of Histone H3 (K4me1).
  • Immunogen:
  • A synthetic lysine4 monomethylated peptide of Histone H3 (K4me1).
  • Host:
  • Rabbit
  • Reactivity:
  • Yeast
  • Specificity:
  • This antibody is expected to react with Histone H3 from yeast to mammals.
  • Form:
  • Liquid
  • Recommend Usage:
  • Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.2 (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Acid treated HeLa extract was treated with a 1 : 500 dilution of antibody Histone H3 (K4me1) polyclonal antibody (Cat # PAB15452) prior to visualization.
  • Dot Blot (Peptide)
  • Dot Blot (Peptide)
  • To confrm the specifcity of the antisera a dot blot system was used with amounts of peptides from 10-250 pmoles.
    Unmodifed, monomethylated, dimethylated and trimethylated peptides surrounding the lysine 4 site on Histone H3 were spotted in lanes 1-4 respectively.
    Lane 5 was also spotted with the monomethylated Lys4 peptide.
    Lanes 6-9 contained peptides starting at residue 6, with the sequence, TARKSTGGKAPRKQLAT, encompassing the residue at lysine 9 on Histone H3 that is unmodifed, monomethylated, dimethylated and trimethylated respectively.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
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