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Last updated: 2017/12/10
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Histon H3 polyclonal antibody

  • Catalog # : PAB15451
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of Histone H3.
  • Immunogen:
  • A synthetic peptide corresponding to C-terminus of Histone H3.
  • Host:
  • Rabbit
  • Reactivity:
  • Yeast
  • Specificity:
  • This antibody is expected to react with Histone H3 from yeast to mammals.
  • Form:
  • Liquid
  • Recommend Usage:
  • Western Blot (1:500-1:1500)
    ChIP (50 ul)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.2 (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • ChIP
  • Western Blot
  • Western Blot
  • 5 uL of acid treated HeLa extracts or recombinant Histone H3 were treated with a 1 : 500 dilution of Histon H3 polyclonal antibody (Cat # PAB15451) prior to visualization.
    Lane 1 : HeLa Extract.
    Lane 2 : HeLa cells treated with sodium butyrate.
    Lane 3 : Recombinant human Histone H3 (0.2 ug).
  • Immunoprecipitation
  • Application Image
  • ChIP
  • Immunoprecipitation
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