Erk1/2 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of Erk1/2.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to 35 amino acids of Erk1/2 with the CGG spacer group added.
Host
Rabbit
Reactivity
Bovine, Chicken, Frog, Fruit fly, Human, Mouse, Rat, Sheep
Specificity
Detects ~44KDa and ~42KDa bands corresponding to the molecular weights of Erk1 and Erk2.
Form
Liquid
Recommend Usage
Flow Cytometry (10 ug/mL)
Immunohistochemistry (1:50)
Western Blot (1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In serum
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Immunohistochemistry
Flow Cytometry
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Publication Reference
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The extracellular signal-regulated kinase: multiple substrates regulate diverse cellular functions.
Yoon S, Seger R.
Growth Factors (Chur, Switzerland) 2006 Mar; 24(1):21.
Application:WB, Rat, PC12 cells.
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Role of reactive oxygen species in angiotensin II-mediated renal growth, differentiation, and apoptosis.
Wolf G.
Antioxidants & Redox Signaling 2005 Sep; 7(9-10):1337.
Application:WB, Human, Cancers, Mammalian cells.
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Purification and properties of extracellular signal-regulated kinase 1, an insulin-stimulated microtubule-associated protein 2 kinase.
Boulton TG, Gregory JS, Cobb MH.
Biochemistry 1991 Jan; 30(1):278.
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The extracellular signal-regulated kinase: multiple substrates regulate diverse cellular functions.
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