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Last updated: 2020/1/26

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Red Blood Cells polyclonal antibody 

  • Catalog # : PAB15378
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against sheep red blood cells.
  • Immunogen:
  • Red blood cells obtained from healthy sheep.
  • Host:
  • Rabbit
  • Reactivity:
  • Sheep
  • Specificity:
  • The total protein is measured using the Biuret procedure, with bovine albumin as a standard. Hemagglutination titer is standardized with an in-house control by measurement of agglutination end-point with 10% sheep red blood cells. Each IgG fraction is tested for purity at a minimum of 40 mg/mL using immunoelectrophoresis. The product is mostly rabbit IgG; no trace of albumin is detected.
    This product is suitable for sensitizing cells in hemolytic complement assays, and for lymphocyte screening procedures, especially screening for T cells and T receptor-bearing lymphocytes. This product is suitable for conjugation with enzymes, radiolabels, fluorochromes or other markers.
  • Form:
  • Lyophilized
  • Preparation Method:
  • The antiserum is prepared by immunizing healthy rabbits with red blood cells obtained from healthy sheep. The IgG fraction is prepared from the specific rabbit antiserum by delipidation, ammonium sulfate fractionation and anion exchange chromatography. The resulting IgG fraction is dialyzed into 0.02 M sodium phosphate, 0.14 M sodium chloride, pH 7.3, 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 um filter, vialed and lyophilized.
  • Recommend Usage:
  • The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Lyophilized from 20 mM sodium phosphate buffer, 140 mM NaCl, pH 7.3 (0.05% sodium azide).
  • Storage Instruction:
  • Store at 4°C on dry atmosphere in lyophilized form.
    After reconstitution with 2.0 mL of deionized or distilled water, store at -20°C or lower for up to one year. Gentle swirling may be used to speed rehydration. Avoid vigorous shaking of the reconstituted material.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Hemolytic Assays
  • Application Image
  • Hemolytic Assays
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