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Last updated: 2021/1/17
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Beta-Galactosidase polyclonal antibody 

  • Catalog # : PAB15374
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against beta-Galactosidase.
  • Immunogen:
  • Recombinant protein corresponding to full length Escherichia coli beta-Galactosidase.
  • Host:
  • Goat
  • Specificity:
  • It shows reactivity to mammalian beta-galactosidase and is tested by Western or dot blot to ensure monospecificity using lysates of D-lac and strain 3.3 of E. coli.
  • Form:
  • Liquid
  • Preparation Method:
  • The antibody is produced by hyperimmunizing goats with the enzyme beta-galactosidase from Escherichia coli. The IgG fraction is prepared by delipidation, ammonium sulfate fractionation, and anion exchange chromatography, and is further purified by solid-phase absorption to remove antibodies to non-beta-galactosidase E. coli proteins. The product is dialyzed into 0.02 M sodium phosphate, 0.14 M sodium chloride, pH 7.3, adjusted to standard titer, filtered through a 0.22 um filter, and vialed. No preservative is added.
  • Isotype:
  • IgG
  • Recommend Usage:
  • ELISA (1:400-1:2000)
    Immunohistochemistry (1:50-1:200)
    Western Blot (1:100-1:500)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM sodium phosphate buffer, 140 mM NaCl, pH 7.3.
  • Storage Instruction:
  • Store at 4°C.
  • Note:
  • Goat IgG fraction to beta-Galactosidase is used in enzyme Immunoassays, fluorescein Immunoassays, blots, cell or tissue immunostaining. Gives sharp precipitation lines in IEP and ID techniques. Suitable for conjugation with enzymes, radiolabels, fluorochromes, or other markers; suitable for attachment to solid supports for use as an immunoadsorbant. It is specially prepared for the detection and purification of recombinant proteins.
  • Applications
  • Western Blot
  • Immunohistochemistry
  • Enzyme Immunoassay
  • Immunodiffusion
  • Application Image
  • Western Blot
  • Immunohistochemistry
  • Enzyme Immunoassay
  • Immunodiffusion
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