This product is suitable for use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. This product is specially prepared for the detection and purification of recombinant proteins. It shows reactivity to beta-galactosidase and is tested by Western or dot blot to ensure monospecificity using lysates of D-lac and strain 3.3 of E. coli.
Immunohistochemistry (1:50-1:200) Western Blot (1:100-1:500) ELISA (1:400-1:2000) The optimal working dilution should be determined by the end user.
In 20 mM sodium phosphate buffer, 140 mM NaCl, pH 7.3
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.