PDK1 polyclonal antibody (DyLight 550)
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PDK1.
Immunogen
A synthetic peptide corresponding to amino acids 300-400 of human PDK1.
Host
Rabbit
Reactivity
Human
Specificity
This antibody reacts with human. No other species have been tested. The immunogen used for this antibody production has 100% homology with mouse, rat, and monkey PDK1 protein. This is specific to PDK1. By Western blot a band is seen at ~49 KDa, representing PDK1 protein. There are also two higher and one lower non-specific molecular weight bands.
Form
Liquid
Conjugation
DyLight 550
Purification
Immunogen affinity purification
Recommend Usage
Western Blot (0.5-2.0 ug/ml)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM sodium borate (0.05% sodium azide).
Storage Instruction
Store at 4°C in the dark.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
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Gene Info — PDK1
Entrez GeneID
5163Protein Accession#
Q15118Gene Name
PDK1
Gene Alias
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Gene Description
pyruvate dehydrogenase kinase, isozyme 1
Omim ID
602524Gene Ontology
HyperlinkGene Summary
Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation. [provided by RefSeq
Other Designations
mitochondrial pyruvate dehydrogenase kinase isoenzyme 1|pyruvate dehydrogenase kinase, isoenzyme 1
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