SELE polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of SELE.
Immunogen
A synthetic peptide corresponding to N-terminus of human SELE.
Host
Rabbit
Theoretical MW (kDa)
65
Reactivity
Bovine, Human, Mouse, Rat
Specificity
This antibody recognizes ~65 KDa of SELE.
Form
Liquid
Purification
Affinity purification
Recommend Usage
Western Blot (0.1-1 ug/mL)
ELISA (0.01-0.1 ug/mL)
Immunoprecipitation (2-5 ug/mL)
Immunohistochemistry (2-5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In TBS, pH 7.2 (BSA, 10% Proclin300)
Storage Instruction
Store at 4°C. For long term storage store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Cell lysate)
The whole cell lysate derived from Jurkat was separated in 10% SDS-PAGE, transferred onto NC membrane, and immunoblotted by SELE polyclonal antibody (Cat # PAB14854) at 1 : 500 . An immunoreactive band around ~65 kDa was observed.Enzyme-linked Immunoabsorbent Assay
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Gene Info — SELE
Entrez GeneID
6401Gene Name
SELE
Gene Alias
CD62E, ELAM, ELAM1, ESEL, LECAM2
Gene Description
selectin E
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is found in cytokine-stimulated endothelial cells and is thought to be responsible for the accumulation of blood leukocytes at sites of inflammation by mediating the adhesion of cells to the vascular lining. It exhibits structural features such as the presence of lectin- and EGF-like domains followed by short consensus repeat (SCR) domains that contain 6 conserved cysteine residues. These proteins are part of the selectin family of cell adhesion molecules. Adhesion molecules participate in the interaction between leukocytes and the endothelium and appear to be involved in the pathogenesis of atherosclerosis. [provided by RefSeq
Other Designations
OTTHUMP00000032745|endothelial adhesion molecule 1|leukocyte endothelial cell adhesion molecule 2
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