CHO cells were transfected with either Vector (pSRa) or expressionvector driving expression of ACP1 (pSRa-LMWPTP), and harvested in Triton X-100 lysis buffer at 72 h after transfection. 2 mg of lysate protein was incubated for 1 h with ACP1 polyclonal antibody (Cat # PAB14684), and immuno-precipitated proteins recovered by Protein A/G were separated on PAGE (12%), and ACP1 detected using a mouse anti-sheep monoclonal antibody.
The product of this gene belongs to the phosphotyrosine protein phosphatase family of proteins. It functions as an acid phosphatase and a protein tyrosine phosphatase by hydrolyzing protein tyrosine phosphate to protein tyrosine and orthophosphate. This enzyme also hydrolyzes orthophosphoric monoesters to alcohol and orthophosphate. This gene is genetically polymorphic, and three common alleles segregating at the corresponding locus give rise to six phenotypes. Each allele appears to encode at least two electrophoretically different isozymes, Bf and Bs, which are produced in allele-specific ratios. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq
acid phosphatase of erythrocyte,adipocyte acid phosphatase,cytoplasmic phosphotyrosyl protein phosphatase,low molecular weight phosphotyrosine protein phosphatase,protein tyrosine phosphatase,red cell acid phosphatase 1