Rabbit polyclonal antibody raised against synthetic peptide of BIRC7.
A synthetic peptide corresponding to amino acids 199-217 of human BIRC7.
Human, Mouse, Rat
Livin is a short-lived protein that is thought to be degraded by the ubiquitin-proteasome pathway. Thus users may want to do a time course when examining the effects of treatments on Livin expression. Users are encouraged to refer to the literature for additional information; for example, Ma et al. 2006. Livin has been shown to undergo proteolytic cleavage during apoptosis. Thus the molecular weight of Livin observed on Western blots may vary depending on the presence of absence of cleavage fragments.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:1000-1:5000) Immunoprecipitation (1:50-1:200) Western Blot (1:1000-1:2000) The optimal working dilution should be determined by the end user.
In serum (0.05% sodium azide)
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Immunohistochemical analysis of BIRC7 in Bouin's-fixed, paraffin-embedded E13.5 mouse embryo (13.5 days post coitum) Bouin's using BIRC7 polyclonal antibody (Cat #PAB14670) at 1 : 2000. The mother was perfused prior to embryo harvesting. A, brain overview. B, brain. C, spinal cord. D, liver and abdominal wall. Hematoxylin-eosin counterstain.
The protein encoded by this gene is a member of the family of inhibitor of apoptosis proteins (IAP) and contains a single copy of a baculovirus IAP repeat (BIR) as well as a RING-type zinc finger domain. The BIR domain is essential for inhibitory activity and interacts with caspases, while the RING finger domain sometimes enhances antiapoptotic activity but does not inhibit apoptosis alone. Two transcript variants encoding different isoforms have been found for this gene. The two isoforms have different antiapoptotic properties, with isoform alpha protecting cells from apoptosis induced by staurosporine and isoform b protecting cells from apoptosis induced by etoposide. [provided by RefSeq
OTTHUMP00000031536,OTTHUMP00000031537,baculoviral IAP repeat-containing protein 7,kidney inhibitor of apoptosis protein,livin inhibitor of apoptosis,livin inhibitor-of-apoptosis,melanoma inhibitor of apoptosis protein