CRYAB polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of CRYAB.
Immunogen
A synthetic peptide corresponding to amino acids 100-175 of human CRYAB.
Host
Rabbit
Reactivity
Chicken, Guinea pig, Human, Mouse, Pig, Primates, Rabbit, Rat, Sheep
Specificity
This antibody is specific to crystallin AB protein.
Form
Liquid
Recommend Usage
Western Blot (2 ug/ mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris-citrate/phosphate buffer, pH 7-8 (0.09% sodium azide)
Storage Instruction
Store at 4°C. Do not freeze.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Detection of CRYAB using CRYAB polyclonal antibody (Cat # PAB14563) (0.5ug/ml).
Lane 1 : Human skeletal muscle.
Lane 2 : Mouse skeletal muscle. -
Gene Info — CRYAB
Entrez GeneID
1410Protein Accession#
P02511Gene Name
CRYAB
Gene Alias
CRYA2, CTPP2, HSPB5
Gene Description
crystallin, alpha B
Gene Ontology
HyperlinkGene Summary
Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (sHSP also known as the HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy. [provided by RefSeq
Other Designations
alpha crystallin B chain|heat-shock 20 kD like-protein
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Interactome
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Disease
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Publication Reference
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Crystallin {gamma}B-I4F mutant protein binds to {alpha}-crystallin and affects lens transparency.
Liu H, Du X, Wang M, Huang Q, Ding L, McDonald HW, Yates JR 3rd, Beutler B, Horwitz J, Gong X.
The Journal of Biological Chemistry 2005 Jul; 280(26):25071.
Application:WB-Ti, Mouse, Lens.
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Small heat shock protein alphaB-crystallin is part of cell cycle-dependent Golgi reorganization.
Gangalum RK, Schibler MJ, Bhat SP.
The Journal of Biological Chemistry 2004 Oct; 279(42):43374.
Application:IF, WB, Human, Rat, Rat hearts, U373MG cells.
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Crystallin {gamma}B-I4F mutant protein binds to {alpha}-crystallin and affects lens transparency.
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