EGLN3 polyclonal antibody
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More Files
- More Functions
Western Blot
Detection of human and rat EGLN3 by Western Blot.
Samples : A. Lysate from cells that were transiently transfected with PHD1 (Lane 1), PHD2 (Lane 2), PHD3 (Lane 3) or PHD4 (Lane 4) constructs or in vitro translated.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of EGLN3.
Immunogen
A synthetic peptide corresponding to amino acids 50-100 of human EGLN3.
Host
Rabbit
Reactivity
Human, Rat
Specificity
This antibody is specific to HIF prolyl hydroxylase 3.
Form
Liquid
Recommend Usage
Western Blot (1:1000-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris-citrate/phosphate buffer, pH 7-8 (0.09% sodium azide)
Storage Instruction
Store at 4°C. Do not freeze.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Detection of human and rat EGLN3 by Western Blot.
Samples : A. Lysate from cells that were transiently transfected with PHD1 (Lane 1), PHD2 (Lane 2), PHD3 (Lane 3) or PHD4 (Lane 4) constructs or in vitro translated. -
Gene Info — EGLN3
Entrez GeneID
112399Protein Accession#
NP_071356.1Gene Name
EGLN3
Gene Alias
FLJ21620, HIFPH3, MGC125998, MGC125999, PHD3
Gene Description
egl nine homolog 3 (C. elegans)
Omim ID
606426Gene Ontology
HyperlinkOther Designations
HIF prolyl hydroxylase 3|egl nine homolog 3|egl nine-like protein 3 isoform
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Interactome
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Pathway
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Disease
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Publication Reference
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PHDs overactivation during chronic hypoxia "desensitizes" HIFalpha and protects cells from necrosis.
Ginouves A, Ilc K, Macias N, Pouyssegur J, Berra E.
PNAS 2008 Mar; 105(12):4745.
Application：WB-Ce, Human, HeLa cells.
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Overexpression and nuclear translocation of hypoxia-inducible factor prolyl hydroxylase PHD2 in head and neck squamous cell carcinoma is associated with tumor aggressiveness.
Jokilehto T, Rantanen K, Luukkaa M, Heikkinen P, Grenman R, Minn H, Kronqvist P, Jaakkola PM.
Clinical Cancer Research 2006 Feb; 12(4):1080.
Application：WB-Tr, Human, HeLa cells.
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Activation of hypoxia-inducible factors in hyperoxia through prolyl 4-hydroxylase blockade in cells and explants of primate lung.
Asikainen TM, Schneider BK, Waleh NS, Clyman RI, Ho WB, Flippin LA, Gunzler V, White CW.
PNAS 2005 Jul; 102(29):10212.
Application：WB, Human, HLMVE-A cells.
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PHDs overactivation during chronic hypoxia "desensitizes" HIFalpha and protects cells from necrosis.
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