Rabbit polyclonal antibody raised against synthetic phosphopeptide of Tph1.
Synthetic phosphopeptide corresponding to residues surrounding S260 of rat Tph1.
Theoretical MW (kDa):
Bovine, Chicken, Dog, Human, Mouse, Rat, Zebra fish
Specific for the ~55 KDa tryptophan hydroxylase protein phosphorylated at Ser260. It is anticipated that This antibody will react with bovine, canine, chicken, mouse and zebra fish based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
Western Blot (1:1000) The optimal working dilution should be determined by the end user.
In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50% glycerol, 10% BSA)
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
Western blot of rat brainstem lysate showing specific immunolabeling of the ~55k Tph1 protein phosphorylated at Ser260. The labeling is specifically blocked by the phosphopeptide (Phos-pep, lane 1) used as antigen. The corresponding non-phosphopeptide (Nonphos-pep, lane 2) did not block the immunolabeling.