GPR81 polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
GPR81 polyclonal antibody (Cat # PAB14397) (0.5g/mL) staining of human liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of GPR81.
Immunogen
A synthetic peptide corresponding to human GPR81.
Sequence
C-QQLARQARMKKATR
Host
Goat
Theoretical MW (kDa)
39.3
Reactivity
Human
Specificity
Approx 40 KDa band observed in human liver lysates (calculated MW of 39.3 KDa according to NP_115943.1). An additional band of unknown identity was also consistently observed at 55 KDa.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:8000)
Western Blot (0.3-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
GPR81 polyclonal antibody (Cat # PAB14397) (0.5g/mL) staining of human liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — GPR81
Entrez GeneID
27198Protein Accession#
NP_115943.1Gene Name
GPR81
Gene Alias
FKSG80, GPR104, TA-GPCR
Gene Description
G protein-coupled receptor 81
Omim ID
606923Gene Ontology
HyperlinkGene Summary
G protein-coupled receptors (GPCRs, or GPRs), such as GPR81, contain 7 transmembrane domains and transduce extracellular signals through heterotrimeric G proteins.[supplied by OMIM
Other Designations
G protein-coupled receptor 104|T-cell activation G protein-coupled receptor|putative chemokine receptor
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Interactome
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Disease
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Publication Reference
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Role of GPR81 in lactate-mediated reduction of adipose lipolysis.
Cai TQ, Ren N, Jin L, Cheng K, Kash S, Chen R, Wright SD, Taggart AK, Waters MG.
Biochemical and Biophysical Research Communications 2008 Dec; 377(3):987.
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Role of GPR81 in lactate-mediated reduction of adipose lipolysis.
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