STK39 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
STK39 polyclonal antibody (Cat # PAB14396) (0.5 ug/mL) staining of Jurkat lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
STK39 polyclonal antibody (Cat # PAB14396) (6 ug/mL) staining of paraffin embedded Human Testis. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of STK39.
Immunogen
A synthetic peptide corresponding to human STK39.
Sequence
C-SQEKSRRVKEENPE
Host
Goat
Theoretical MW (kDa)
59.5
Reactivity
Human
Specificity
Approx 65 KDa band observed in human brain (cerebellum) lysates and in lysates of cell line Jurkat (calculated MW of 59.5 KDa according to NP_037365.2).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:4000)
Immunohistochemistry (6 ug/mL)
Western Blot (0.3-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 0.5 mg/mL Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
STK39 polyclonal antibody (Cat # PAB14396) (0.5 ug/mL) staining of Jurkat lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
STK39 polyclonal antibody (Cat # PAB14396) (6 ug/mL) staining of paraffin embedded Human Testis. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — STK39
Entrez GeneID
27347Protein Accession#
NP_037365.2Gene Name
STK39
Gene Alias
DCHT, DKFZp686K05124, PASK, SPAK
Gene Description
serine threonine kinase 39 (STE20/SPS1 homolog, yeast)
Omim ID
607648Gene Ontology
HyperlinkGene Summary
This gene encodes a serine/threonine kinase that is thought to function in the cellular stress response pathway. The kinase is activated in response to hypotonic stress, leading to phosphorylation of several cation-chloride-coupled cotransporters. The catalytically active kinase specifically activates the p38 MAP kinase pathway, and its interaction with p38 decreases upon cellular stress, suggesting that this kinase may serve as an intermediate in the response to cellular stress. [provided by RefSeq
Other Designations
Ste20-like protein kinase|proline-alanine-rich STE20-related kinase|small intestine SPAK-like kinase
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Interactome
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Disease
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Publication Reference
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An analysis of candidate autism loci on chromosome 2q24-q33: evidence for association to the STK39 gene.
Ramoz N, Cai G, Reichert JG, Silverman JM, Buxbaum JD.
American Journal of Medical Genetics. Part B, Neuropsychiatric Genetics 2008 Oct; 147B(7):1152.
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An analysis of candidate autism loci on chromosome 2q24-q33: evidence for association to the STK39 gene.
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