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Last updated: 2017/11/12

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STK39 polyclonal antibody

  • Catalog # : PAB14396
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of STK39.
  • Immunogen:
  • A synthetic peptide corresponding to human STK39.
  • Sequence:
  • C-SQEKSRRVKEENPE
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 59.5
  • Reactivity:
  • Human
  • Specificity:
  • Approx 65 KDa band observed in human brain (cerebellum) lysates and in lysates of cell line Jurkat (calculated MW of 59.5 KDa according to NP_037365.2).
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Recommend Usage:
  • ELISA (1:8000)
    Western Blot (0.3-1 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 0.5 mg/mL Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • STK39 polyclonal antibody (Cat # PAB14396) (0.5 ug/mL) staining of Jurkat lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • STK39 polyclonal antibody (Cat # PAB14396) (3.8 ug/mL) staining of paraffin embedded human cerebral cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Gene Name:
  • STK39
  • Gene Alias:
  • DCHT,DKFZp686K05124,PASK,SPAK
  • Gene Description:
  • serine threonine kinase 39 (STE20/SPS1 homolog, yeast)
  • Gene Summary:
  • This gene encodes a serine/threonine kinase that is thought to function in the cellular stress response pathway. The kinase is activated in response to hypotonic stress, leading to phosphorylation of several cation-chloride-coupled cotransporters. The catalytically active kinase specifically activates the p38 MAP kinase pathway, and its interaction with p38 decreases upon cellular stress, suggesting that this kinase may serve as an intermediate in the response to cellular stress. [provided by RefSeq
  • Other Designations:
  • Ste20-like protein kinase,proline-alanine-rich STE20-related kinase,small intestine SPAK-like kinase
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