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NKX2-5 polyclonal antibody

  • Catalog # : PAB14394
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of NKX2-5.
  • Immunogen:
  • A synthetic peptide corresponding to human NKX2-5.
  • Sequence:
  • C-PRAYSDPDPAKDPR
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 34.9, 11.7, 16.1
  • Reactivity:
  • Human, Mouse, Rat
  • Specificity:
  • Approx 45 KDa band observed in human heart lysates (calculated MW of 34.9 KDa according to NP_004378.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Shiojima et al, Circ Res. 1996 Nov;79(5):920-9.; PMID: 8888684).
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Recommend Usage:
  • ELISA (1:32000)
    Western Blot (0.3-1 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • NKX2-5 polyclonal antibody (Cat # PAB14394) (0.3 ug/mL) staining of human heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • ELISA
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 1482
  • Protein Accession#:
  • NP_004378.1;NP_001159647.1;NP_001159648.1
  • Gene Name:
  • NKX2-5
  • Gene Alias:
  • CHNG5,CSX,CSX1,NKX2.5,NKX2E,NKX4-1
  • Gene Description:
  • NK2 transcription factor related, locus 5 (Drosophila)
  • Gene Summary:
  • Homeobox-containing genes play critical roles in regulating tissue-specific gene expression essential for tissue differentiation, as well as determining the temporal and spatial patterns of development (Shiojima et al., 1995 [PubMed 7665173]). It has been demonstrated that a Drosophila homeobox-containing gene called 'tinman' is expressed in the developing dorsal vessel and in the equivalent of the vertebrate heart. Mutations in tinman result in loss of heart formation in the embryo, suggesting that tinman is essential for Drosophila heart formation. Furthermore, abundant expression of Csx, the presumptive mouse homolog of tinman, is observed only in the heart from the time of cardiac differentiation. CSX, the human homolog of murine Csx, has a homeodomain sequence identical to that of Csx and is expressed only in the heart, again suggesting that CSX plays an important role in human heart formation.[supplied by OMIM
  • Other Designations:
  • NK2 transcription factor homolog E,NK2 transcription factor related, locus 5,cardiac-specific homeo box,tinman paralog
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