CCT3 polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
CCT3 polyclonal antibody (Cat # PAB14393) (0.01 ug/mL) staining of mouse testis lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of CCT3.
Immunogen
A synthetic peptide corresponding to human CCT3.
Sequence
C-GHKKKGDDQSRQGG
Host
Goat
Theoretical MW (kDa)
60.5, 60.5, 56.4
Reactivity
Mouse, Rat
Specificity
Approx 65 KDa band observed in mouse and rat testis lysates (calculated MW of 60.5 KDa according to human NP_005989.3 and 60.6KDa according to Mouse NP_033966.1 and Rat NP_954522.1).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:32000)
Western Blot (0.01-0.03 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
CCT3 polyclonal antibody (Cat # PAB14393) (0.01 ug/mL) staining of mouse testis lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — CCT3
Entrez GeneID
7203Protein Accession#
NP_005989.3;NP_001008883.1;NP_001008800.1.Gene Name
CCT3
Gene Alias
CCT-gamma, CCTG, PIG48, TCP-1-gamma, TRIC5
Gene Description
chaperonin containing TCP1, subunit 3 (gamma)
Omim ID
600114Gene Ontology
HyperlinkGene Summary
This gene encodes a molecular chaperone that is member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq
Other Designations
OTTHUMP00000025735|T-complex protein 1, gamma subunit|TCP1 (t-complex-1) ring complex, polypeptide 5|chaperonin containing TCP1, subunit 3
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Interactome
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Disease
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Publication Reference
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Toward a confocal subcellular atlas of the human proteome.
Barbe L, Lundberg E, Oksvold P, Stenius A, Lewin E, Bjorling E, Asplund A, Ponten F, Brismar H, Uhlen M, Andersson-Svahn H.
Molecular & Cellular Proteomics: MCP 2008 Mar; 7(3):499.
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Toward a confocal subcellular atlas of the human proteome.
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