Goat polyclonal antibody raised against synthetic peptide of CYBB.
A synthetic peptide corresponding to human CYBB.
Theoretical MW (kDa):
Approx 85 KDa band observed in human peripheral blood mononucleocytes ysates (calculated MW of 65.3KDa according to NP_000388.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Teahan et al, Nature. 1987 Jun 25-Jul 1;327(6124):720-1. PMID: 3600769).
Antigen affinity purification
ELISA (1:2000) Western Blot (0.03-0.1 ug/mL) The optimal working dilution should be determined by the end user.
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
CYBB polyclonal antibody (Cat # PAB14389) (0.03 ug/mL) staining of human peripheral blood mononucleocyte lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Cytochrome b (-245) is composed of cytochrome b alpha (CYBA) and beta (CYBB) chain. It has been proposed as a primary component of the microbicidal oxidase system of phagocytes. CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD). In this disorder, there is decreased activity of phagocyte NADPH oxidase; neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles. The cause of the killing defect is an inability to increase the cell's respiration and consequent failure to deliver activated oxygen into the phagocytic vacuole. [provided by RefSeq