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Last updated: 2019/8/18

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Nitrotyrosine polyclonal antibody (Biotin) 

  • Catalog # : PAB14352
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against Nitrotyrosine.
  • Immunogen:
  • Nitrotyrosine conjugated with KLH.
  • Host:
  • Goat
  • Specificity:
  • Specifically binds to nitro-LDL and other nitrated proteins. The antibody can be used for detection of nitrotyrosine modified protein in plasma andother nitrated proteins, immunoassays and immunoblots.
  • Form:
  • Lyophilized
  • Conjugation:
  • Biotin
  • Recommend Usage:
  • ELISA (1:200-1:20000)
    Western Blot (1:200-1:20000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Lyophilized from 75 mM Sodium Phosphate, 75 mM NaCl, pH 7.2 (10 mg/ml BSA, 0.5 mM EDTA, 0.02% sodium azide).
  • Storage Instruction:
  • Store at 4°C on dry atmosphere.
    After reconstitution with 500 uL deionized water, store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • Lyophilized product should be stored refrigerated until opened. After opening, restore to 500 uL with distilled water. If it is not completely clear after standing for 1-2 hours at room temperature, centrifuge the product. It is stable for several weeks at 4°C as an undiluted liquid. Do not use for more than one day after dilution. For extended storage after reconstitution, we suggest aliquot to avoid repeated freezing and thawing; or the addition of an equal volume of glycerol to make a final glycerol concentration of 50%, followed by storage at -20°C. The concentration of protein and buffer salts will decrease to one-half of the original after the addition of glycerol.
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot
  • ELISA
  • Application Image
  • Western Blot
  • ELISA
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