Ngf polyclonal antibody
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Immunohistochemistry
Immunohistochemical staining of nerve growth factor (Ngf) in rat cervical ganglion using Ngf polyclonal antibody (Cat # PAB14337).
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Specification
Product Description
Rabbit polyclonal antibody raised against native Ngf.
Immunogen
Native purified Ngf from mouse submaxillary salivary gland.
Host
Rabbit
Reactivity
Birds, Human, Mouse, Rat
Specificity
A cross reactivity of less than 1% to recombinant human BDNF, NT3, NT4/5 by ELISA has been shown.
Form
Lyophilized
Recommend Usage
Western Blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from PBS
Storage Instruction
Store at 4°C on dry atmosphere.
After reconstitution with deionized water, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Immunohistochemistry
Immunohistochemical staining of nerve growth factor (Ngf) in rat cervical ganglion using Ngf polyclonal antibody (Cat # PAB14337).Enzyme-linked Immunoabsorbent Assay
Inhibition Assay
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Gene Info — Ngf
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Publication Reference
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An improved procedure for the immunohistochemical localization of nerve growth factor-like immunoreactivity.
X F Zhou, C Zettler, R A Rush.
Journal of Neuroscience Methods 1994 Sep; 54(1):95.
Application：IHC-Fr, WB-Re, Rat, Rat superior cervical ganglia, Recombinant proteins.
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Characterization of antibodies to synthetic nerve growth factor (NGF) and proNGF peptides.
Ebendal T, Persson H, Larhammar D, Lundstromer K, Olson L.
Journal of Neuroscience Research 1989 Mar; 22(3):223.
Application：ELISA, IA, IF, IHC-Fr, Mouse, Mouse salivary glands, Peptides.
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The nerve growth factor (NGF): chemical properties and metabolic effects.
Angeletti PU, Levi-Montalcini R, Calissano P.
Advances in Enzymology and Related Areas of Molecular Biology 1968 Jan; 31:51.
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An improved procedure for the immunohistochemical localization of nerve growth factor-like immunoreactivity.
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