AP1B1 polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
Western blot analysis on human brain lysate under reducing conditions using AP1B1 polyclonal antibody (Cat # PAB14335) at a dilution of 1 : 100. This antibody labels a major protein of approximately 100 KDa of molecular weight and several minor proteins around 70 KDa in both rat and human tissue. The presence of the two lower bands (IgG fragments) is due to the magnetic bead purification method employed.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of AP1B1.
Immunogen
A synthetic peptide (conjugated with diphtheria toxoid) corresponding to amino acids 11-29 of human AP1B1.
Sequence
KKGEIFELKAELNSDKKEK
Host
Rabbit
Reactivity
Human, Rat
Specificity
This antibody has been shown to be specific to AP-1 complex subunit beta-1.
Form
Lyophilized
Recommend Usage
Immunohistochemistry (1:1000-1:5000)
Western Blot (1:100-1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from PBS
Storage Instruction
Store at 4°C on dry atmosphere.
After reconstitution with deionized water, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Tissue lysate)
Western blot analysis on human brain lysate under reducing conditions using AP1B1 polyclonal antibody (Cat # PAB14335) at a dilution of 1 : 100. This antibody labels a major protein of approximately 100 KDa of molecular weight and several minor proteins around 70 KDa in both rat and human tissue. The presence of the two lower bands (IgG fragments) is due to the magnetic bead purification method employed.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Frozen sections)
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Gene Info — AP1B1
Entrez GeneID
162Protein Accession#
Q10567;O35643;P52303;Q2KJB2Gene Name
AP1B1
Gene Alias
ADTB1, AP105A, BAM22, CLAPB2
Gene Description
adaptor-related protein complex 1, beta 1 subunit
Omim ID
600157Gene Ontology
HyperlinkGene Summary
Adaptor protein complex 1 is found at the cytoplasmic face of coated vesicles located at the Golgi complex, where it mediates both the recruitment of clathrin to the membrane and the recognition of sorting signals within the cytosolic tails of transmembrane receptors. This complex is a heterotetramer composed of two large, one medium, and one small adaptin subunit. The protein encoded by this gene serves as one of the large subunits of this complex and is a member of the adaptin protein family. This gene is a candidate meningioma gene. Two transcript variants encoding different isoforms have been found for this gene, and variants utilizing alternative polyadenylation signals exist. [provided by RefSeq
Other Designations
Golgi adaptor HA1/AP1 adaptin beta subunit|adaptor protein complex AP-1 beta 1 subunit|adaptor-related protein complex 1 beta 1 subunit|beta-adaptin 1|beta-prime-adaptin|clathrin assembly protein complex 1 beta large chain|plasma membrane adaptor HA2/AP2
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Interactome
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Pathway
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Disease
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Publication Reference
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Endogenously nitrated proteins in mouse brain: links to neurodegenerative disease.
Sacksteder CA, Qian WJ, Knyushko TV, Wang H, Chin MH, Lacan G, Melega WP, Camp DG 2nd, Smith RD, Smith DJ, Squier TC, Bigelow DJ.
Biochemistry 2006 Jul; 45(26):8009.
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Characterization of the mouse beta-prime adaptin gene; cDNA sequence, genomic structure, and chromosomal localization.
Guilbaud C, Peyrard M, Fransson I, Clifton SW, Roe BA, Carter NP, Dumanski JP.
Mammalian Genome 1997 Sep; 8(9):651.
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Structure of the promoter and genomic organization of the human beta'-adaptin gene (BAM22) from chromosome 22q12.
Peyrard M, Pan HQ, Kedra D, Fransson I, Swahn S, Hartman K, Clifton SW, Roe BA, Dumanski JP.
Genomics 1996 Aug; 36(1):112.
Application：IHC, WB-Ti, Human, Human meningiomas.
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Endogenously nitrated proteins in mouse brain: links to neurodegenerative disease.
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