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Last updated: 2017/4/23
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MPO polyclonal antibody

  • Catalog # : PAB14328
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against native MPO.
  • Immunogen:
  • Native purified human polymorphonuclear leucocytes MPO.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Specificity:
  • This antiserum reacts with human myeloperoxidase.
  • Form:
  • Lyophilized
  • Recommend Usage:
  • Western Blot (1:500-1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Lyophilized from antiserum
  • Storage Instruction:
  • Store at 4°C on dry atmosphere.
    After reconstitution with 250 uL of deionized water, centrifuge to remove any insoluble material and store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Publication Reference
  • Applications
  • Western Blot
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Frozen sections)
  • Immunocytochemistry
  • Immunofluorescence
  • Immunofluorescence
  • Staining of Myeloperoxidase (MPO) in acetone fixed human neutrophils. Cytospin slides of human neutrophils isolated from peripheral blood were fixed by immersion in cold acetone for 90 seconds and blocked with 10% FCS prior to incubation with MPO polyclonal antibody (Cat # PAB14328). The antibody was diluted to 1 : 1000 and incubated at room temperature for 1h and visualized with FITC-labeled secondary antibody.
  • Application Image
  • Western Blot
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Frozen sections)
  • Immunocytochemistry
  • Gene Information
  • Entrez GeneID:
  • 4353
  • Gene Name:
  • MPO
  • Gene Alias:
  • -
  • Gene Description:
  • myeloperoxidase
  • Gene Summary:
  • Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of netrophils. [provided by RefSeq
  • Other Designations:
  • -
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