Staining of Myeloperoxidase (MPO) in acetone fixed human neutrophils. Cytospin slides of human neutrophils isolated from peripheral blood were fixed by immersion in cold acetone for 90 seconds and blocked with 10% FCS prior to incubation with MPO polyclonal antibody (Cat # PAB14328). The antibody was diluted to 1 : 1000 and incubated at room temperature for 1h and visualized with FITC-labeled secondary antibody.
Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of netrophils. [provided by RefSeq