DBH polyclonal antibody
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Immunohistochemistry
Immunohistochemical staining of Dopamine beta-Hydroxylase (DBH) in guinea pig brain stem using DBH polyclonal antibody (Cat # PAB14325).
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Specification
Product Description
Rabbit polyclonal antibody raised against native DBH.
Immunogen
Native purified DBH from bovine adrenal glands.
Host
Rabbit
Reactivity
Bovine, Guinea pig, Human, Rat
Specificity
This antiserum has been extensively characterised in several laboratories. It reacts with a single band on western blot of bovine adrenal homogenates and stains only cells known to contain DBH, such as sympathetic neurons, adrenal medullary cells and central adrenergic neurons. Staining is abolished by preincubation with the enzyme.
Form
Lyophilized
Recommend Usage
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from PBS
Storage Instruction
Store at 4°C on dry atmosphere.
After reconstitution with deionized water, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Immunohistochemistry
Immunohistochemical staining of Dopamine beta-Hydroxylase (DBH) in guinea pig brain stem using DBH polyclonal antibody (Cat # PAB14325).Radioimmunoassay
Inhibition Assay
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Gene Info — DBH
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Publication Reference
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Electron immunocytochemical localization of enkephalin-like material in catecholamine-containing cells of the carotid body, the adrenal medulla, and in pheochromocytomas of man and other mammals.
Varndell IM, Tapia FJ, De Mey J, Rush RA, Bloom SR, Polak JM.
The Journal of Histochemistry and Cytochemistry 1982 Jul; 30(7):682.
Application：IEM, Cat, Cat adrenal medulla, Cat carotid body.
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Dopamine beta-hydroxylase in health and disease.
Rush RA, Geffen LB.
Crit Rev Clin Lab Sci 1980 Jan; 12(3):241.
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Electron immunocytochemical localization of enkephalin-like material in catecholamine-containing cells of the carotid body, the adrenal medulla, and in pheochromocytomas of man and other mammals.
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