MBP polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of MBP.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to amino acids 68-86 of guinea pig MBP.
Sequence
YGSLPQKSQRSQDENPVV
Host
Rabbit
Reactivity
Guinea pig, Human, Rat
Specificity
This antiserum recognizes MBP in demyelinated nerve tissues. Immunohistochemical analysis of lesioned rat spinal cord indictaes a high level of specificity for this antiserum.
Form
Lyophilized
Recommend Usage
Immunohistochemistry (1:1000-1:4000)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from PBS
Storage Instruction
Store at 4°C on dry atmosphere.
After reconstitution with deionized water, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing. -
Applications
Immunohistochemistry
Immunohistochemistry on human brain with brain trauma (car accident), using MBP polyclonal antibody (Cat # PAB14324) at a dilution of 1 : 2000. Courtesy of Dr. Wei-Ping Gai, The Flinders University of South Australia. -
Publication Reference
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Myelin basic protein-diverse conformational states of an intrinsically unstructured protein and its roles in myelin assembly and multiple sclerosis.
Harauz G, Ishiyama N, Hill CM, Bates IR, Libich DS, Fares C.
Micron 2004 Oct; 35(7):503.
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T cell-based therapeutic vaccination for spinal cord injury.
Schwartz M, Hauben E.
Progress in Brain Research 2002 Jan; 137:401.
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Posttraumatic therapeutic vaccination with modified myelin self-antigen prevents complete paralysis while avoiding autoimmune disease.
Hauben E, Agranov E, Gothilf A, Nevo U, Cohen A, Smirnov I, Steinman L, Schwartz M.
J Clin Invest 2001 Aug; 108(4):591.
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Myelin basic protein-diverse conformational states of an intrinsically unstructured protein and its roles in myelin assembly and multiple sclerosis.
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