Cntn6 polyclonal antibody
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More Files
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Immunohistochemistry (Frozen sections)
Immunohistochemical detection of Cntn6 in rat sciatic nerve. Anesthetized animals were perfused with 4% paraformaldehyde in PBS containing 12.5% picric acid. Floating cryostat sections of sciatic nerve were permeabilized with 0.5% NP-40 in Tris-buffered saline and blocked with 5% normal goat serum. Sections were incubated with Cntn6 polyclonal antibody (Cat # PAB14323) at 1 : 3000, followed by incubation with biotinylated sheep anti-rabbit antibody and avidin-biotin-peroxidase complex. The immunocomplex was visualized with DAB.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of Cntn6.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to amino acids 54-64 of mouse Cntn6.
Sequence
GYPSPHYRWKQ
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antiserum stains beautifully the NB3 in DRG, where it nicely stains a subpopulation of neurons and in the trigeminal nerve root, where staining is restricted to the oligodendrocytes.
Form
Lyophilized
Recommend Usage
Immunohistochemistry (1:1000-1:4000)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from antiserum.
Storage Instruction
Store at 4°C on dry atmosphere.
After reconstitution with deionized water, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing. -
Applications
Immunohistochemistry (Frozen sections)
Immunohistochemical detection of Cntn6 in rat sciatic nerve. Anesthetized animals were perfused with 4% paraformaldehyde in PBS containing 12.5% picric acid. Floating cryostat sections of sciatic nerve were permeabilized with 0.5% NP-40 in Tris-buffered saline and blocked with 5% normal goat serum. Sections were incubated with Cntn6 polyclonal antibody (Cat # PAB14323) at 1 : 3000, followed by incubation with biotinylated sheep anti-rabbit antibody and avidin-biotin-peroxidase complex. The immunocomplex was visualized with DAB. -
Gene Info — Cntn6
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Publication Reference
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NB-3/Notch1 pathway via Deltex1 promotes neural progenitor cell differentiation into oligodendrocytes.
Cui XY, Hu QD, Tekaya M, Shimoda Y, Ang BT, Nie DY, Sun L, Hu WP, Karsak M, Duka T, Takeda Y, Ou LY, Dawe GS, Yu FG, Ahmed S, Jin LH, Schachner M, Watanabe K, Arsenijevic Y, Xiao ZC.
The Journal of Biological Chemistry 2004 Jun; 279(24):25858.
Application：IF, IP, WB, Rat, Rat striatum.
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Impaired motor coordination in mice lacking neural recognition molecule NB-3 of the contactin/F3 subgroup.
Takeda Y, Akasaka K, Lee S, Kobayashi S, Kawano H, Murayama S, Takahashi N, Hashimoto K, Kano M, Asano M, Sudo K, Iwakura Y, Watanabe K.
Journal of Neurobiology 2003 Sep; 56(3):252.
Application：IHC-P, WB-Ti, Mouse, Rat, Brains.
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Expression and regulation of a gene encoding neural recognition molecule NB-3 of the contactin/F3 subgroup in mouse brain.
Lee S, Takeda Y, Kawano H, Hosoya H, Nomoto M, Fujimoto D, Takahashi N, Watanabe K.
Gene 2000 Mar; 245(2):253.
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NB-3/Notch1 pathway via Deltex1 promotes neural progenitor cell differentiation into oligodendrocytes.
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