Saporin polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against Saporin.
Immunogen
Whole Saporin molecule.
Host
Rabbit
Specificity
Confirmed to react with purified saporin.
Form
Lyophilized
Recommend Usage
Western Blot (1:200-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from PBS
Storage Instruction
Store at 4°C on dry atmosphere.
After reconstitution with deionized water, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Western blot analysis using Saporin polyclonal antibody (Cat # PAB14312). HRP-labelled sheep anti-rabbit was used as the secondary antibody and the immunocomplex was then visualised by DAB. The expected molecular weight of the purified saporin is 29.5 KDa. SC : Spinal Cord homogenate (rat).Immunohistochemistry
Immunofluorescence
Enzyme-linked Immunoabsorbent Assay
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Publication Reference
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Polynucleotide:Adenosine glycosidase is the sole activity of ribosome-inactivating proteins on DNA.
Barbieri L, Valbonesi P, Righi F, Zuccheri G, Monti F, Gorini P, Samori B, Stirpe F.
Journal of Biochemistry 2000 Nov; 128(5):883.
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The crystal structure of saporin SO6 from Saponaria officinalis and its interaction with the ribosome.
Savino C, Federici L, Ippoliti R, Lendaro E, Tsernoglou D.
FEBS Letters 2000 Mar; 470(3):239.
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Crystallization and preliminary X-ray study of saporin, a ribosome-inactivating protein from Saponaria officinalis.
Savino C, Federici L, Brancaccio A, Ippoliti R, Lendaro E, Tsernoglou D.
Acta Crystallographica. Section D, Biological Crystallography 1998 Jul; 54(Pt 4):636.
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Polynucleotide:Adenosine glycosidase is the sole activity of ribosome-inactivating proteins on DNA.
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