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Last updated: 2022/5/22
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BDNF polyclonal antibody 

  • Catalog # : PAB14311
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against recombinant BDNF.
  • Immunogen:
  • Recombinant protein corresponding to human BDNF.
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Mouse, Rat
  • Specificity:
  • This antibody has less than 0.1% cross-reactivity against NGF, recombinant NT3 and NT4 by dot blot.
  • Form:
  • Lyophilized
  • Purification:
  • Protein G purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • Immunohistochemistry (1-10 ug/mL)
    ELISA (0.1 ug/mL)
    Inhibition Assay (1-10 ug/mL)
    Western Blot (1-10 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Reconstitute in 500 uL sterile-filtered 1X PBS, pH 7.2-7.6. Glycerol (1:1) may be added for an additional stability.
  • Storage Instruction:
  • Store at 4°C on dry atmosphere. After reconstitution store at -20°C
    Aliquot to avoid repeated freezing and thawing.
  • Publication Reference
  • Applications
  • Western Blot
  • Western Blot
  • Western Blot analysis of (1) BDNF of rhBDNF R-088-100 (whole serum), (2) BDNF-isoform of rhBDNF R-088-100 (whole serum), (3) SHSY5Y of rhBDNF R-088-100 (whole serum), (4) Human brain of rhBDNF R-088-100 (whole serum), (5) Human brain of R-017-500 (IgG, 10 ug/mL), monomeric BDNF at 14 kDa and proBDNF is detected at the expected molecular weight of 32 kDa for glycosylated proBDNF monomer.
  • Immunohistochemistry
  • Immunohistochemistry
  • Immunohistochemical staining for BDNF in rat dorsal root ganglion (DRG) using BDNF polyclonal antibody (Cat # PAB14311) at an amount of 5 ug/mL . Neuron cell bodies are strongly stained for BDNF.
  • Enzyme-linked Immunoabsorbent Assay
  • Inhibition Assay
  • Application Image
  • Enzyme-linked Immunoabsorbent Assay
  • Inhibition Assay
  • Gene Information
  • Entrez GeneID:
  • 627
  • Gene Name:
  • BDNF
  • Gene Alias:
  • MGC34632
  • Gene Description:
  • brain-derived neurotrophic factor
  • Gene Summary:
  • The protein encoded by this gene is a member of the nerve growth factor family. It is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of this gene is reduced in both Alzheimer's and Huntington disease patients. This gene may play a role in the regulation of stress response and in the biology of mood disorders. Multiple transcript variants encoding distinct isoforms have been described for this gene. [provided by RefSeq
  • Other Designations:
  • neurotrophin
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