The cryostat section of the sheep hypothalamus was incubated in OXT polyclonal antibody (Cat # PAB14310) at the dilution of 1 : 1000 overnight followed by incubation with biotinylated secondary antibodies. Cell bodies and nerve terminals (from cells that produce this hormone) in the sheep brain are intensely stained. This figure shows staining of cells when no pre-absorption is performed.
There are two proteins encoded by this gene, oxytocin and neurophysin I. Oxytocin is posterior pituitary hormone which is synthesized as an inactive precursor in the hypothalamus along with its carrier protein neurophysin I. Together with neurophysin, it is packaged into neurosecretory vesicles and transported axonally to the nerve endings in the neurohypophysis, where it is either stored or secreted into the bloodstream. The precursor seems to be activated while it is being transported along the axon to the posterior pituitary. This hormone contracts smooth muscle during parturition and lactation. It is also involved in cognition, tolerance, adaptation and complex sexual and maternal behaviour, as well as in the regulation of water excretion and cardiovascular functions. [provided by RefSeq
OTTHUMP00000030079,neurophysin I,oxytocin, prepro- (neurophysin I),oxytocin-neurophysin I,oxytocin-neurophysin I, preproprotein