Rabbit polyclonal antibody raised against synthetic peptide of Rel.
A synthetic peptide (conjugated with KLH) corresponding to Drosophila Rel.
Flies, Fruit fly
This antibody detects specifically target peptide derived from Drosophila Relish by ELISA and Dot Blotting. Also detects cleaved and uncleaved Relish in PFA-fixed Drosphila tissue (see image). Cross reactivity with human, mouse and rat have not been tested.
ELISA (1:1000-1:5000) Immunohistochemistry (1:50) The optimal working dilution should be determined by the end user.
1x PBS (pH7.4)
Store at -20°C. For long term storage store at -80°C. Aliquot after reconstitution to avoid repeated freezing and thawing.
Third instar larva were parenterally challenged with Erwinia carotovora (a natural fly pathogen). After 2 hours, the larval fat body was dissected, fixed with paraformaldehyde, and immunostained with Rel polyclonal antibody (Cat # PAB14193) (1/50 dilution), followed by secondary incubation with Goat anti-rabbit-Cy5. Tissues were also stained with SYTO24 DNA stain. Images captured by confocal microscopy.