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Last updated: 2017/11/19
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NAMPT polyclonal antibody

  • Catalog # : PAB14190
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of NAMPT.
  • Immunogen:
  • A synthetic peptide corresponding to human NAMPT.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Specificity:
  • The rabbit anti-human Visfatin antibody detects specifically target derived from human recombinant protein as well as cell lysates of MD231 and Hela at various concentrations. Cross reactivity with mouse and rat are expected from sequence similarity.
  • Form:
  • Lyophilized
  • Recommend Usage:
  • ELISA (1:1000000)
    Western Blot (1:1000-1:5000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Lyophilized from PBS
  • Storage Instruction:
  • Store at -20°C. For long term storage store at -80°C.
    Aliquot after reconstitution to avoid repeated freezing and thawing.
  • Publication Reference
  • 2.
  • Visfatin: a protein secreted by visceral fat that mimics the effects of insulin.
    Fukuhara A, Matsuda M, Nishizawa M, Segawa K, Tanaka M, Kishimoto K, Matsuki Y, Murakami M, Ichisaka T, Murakami H, Watanabe E, Takagi T, Akiyoshi M, Ohtsubo T, Kihara S, Yamashita S, Makishima M, Funahashi T, Yamanaka S, Hiramatsu R, Matsuzawa Y, Shimomura I.Science. 2005 Jan 21;307(5708):426-30. Epub 2004 Dec 16.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Representative blot from a previous lot.
    Lane 1. protein marker.
    Lane 2. Reference control.
    Lane 3-4. Positive control, HeLa and MD231 whole cell lysate.
    Lane 5. Negative control.
    The membrane blot was probed with NAMPT polyclonal antibody (Cat # PAB14190) (1 ug/mL).
    Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system.
    Arrows indicate cellular NAMPT from human and mouse cells (52 KDa).
  • ELISA
  • Enzyme Immunoassay
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • ELISA
  • Enzyme Immunoassay
  • Gene Information
  • Gene Name:
  • NAMPT
  • Gene Alias:
  • 1110035O14Rik,DKFZp666B131,MGC117256,PBEF,PBEF1,VF,VISFATIN
  • Gene Description:
  • nicotinamide phosphoribosyltransferase
  • Gene Summary:
  • This gene encodes a protein that catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein is an adipokine that is localized to the bloodstream and has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It also activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity. This gene has a pseudogene on chromosome 10. [provided by RefSeq
  • Other Designations:
  • NAmPRTase,pre-B cell-enhancing factor,pre-B-cell colony enhancing factor 1
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