NEFH polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Strip blot of crude rat spinal extract stained with NEFH polyclonal antibody (Cat # PAB14170). A prominent band at an apparent SDS-PAGE molecular weight of 200 KDa corresponds to phosphorylated NF-H.
Immunofluorescence
A section of rat spinal cord stained with NEFH polyclonal antibody (Cat # PAB14170) (green) and ubiquitin C-terminal hydrolase 1 monoclonal antibody (UCHL1) clone MCA-BH7 (red).
The NEFH polyclonal antibody binds primarily to phosphorylated axonal forms of NEFH, and so stains axons coursing between the large UCHL1 positive neurons.
These large cells are a-motorneurons and UCHL1 protein is a major component of the perikarya and dendrites of these cells.
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Specification
Product Description
Rabbit polyclonal antibody raised against NEFH.
Immunogen
Bovine NEFH.
Host
Rabbit
Theoretical MW (kDa)
200-220
Reactivity
Bovine
Form
Liquid
Recommend Usage
Western Blot (1:10000)
Immunofluorescence (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In serum (5 mM sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Strip blot of crude rat spinal extract stained with NEFH polyclonal antibody (Cat # PAB14170). A prominent band at an apparent SDS-PAGE molecular weight of 200 KDa corresponds to phosphorylated NF-H.Immunohistochemistry
Immunofluorescence
A section of rat spinal cord stained with NEFH polyclonal antibody (Cat # PAB14170) (green) and ubiquitin C-terminal hydrolase 1 monoclonal antibody (UCHL1) clone MCA-BH7 (red).
The NEFH polyclonal antibody binds primarily to phosphorylated axonal forms of NEFH, and so stains axons coursing between the large UCHL1 positive neurons.
These large cells are a-motorneurons and UCHL1 protein is a major component of the perikarya and dendrites of these cells.Enzyme-linked Immunoabsorbent Assay
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Publication Reference
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Hyperphosphorylated neurofilament NF-H is a serum biomarker of axonal injury.
Shaw G, Yang C, Ellis R, Anderson K, Parker Mickle J, Scheff S, Pike B, Anderson DK, Howland DR.
Biochemical and Biophysical Research Communications 2005 Nov; 336(4):1268.
Application：ELISA, IF, WB-Ti, Rat, Spinal cord, Neuron/glial cells, Serum.
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Hyperphosphorylated neurofilament NF-H is a serum biomarker of axonal injury.
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