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Last updated: 2017/6/25
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NEFH polyclonal antibody

  • Catalog # : PAB14170
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against NEFH.
  • Immunogen:
  • Bovine NEFH.
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 200-220
  • Reactivity:
  • Bovine
  • Form:
  • Liquid
  • Recommend Usage:
  • Western Blot (1:10000)
    Immunofluorescence (1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In serum (5 mM sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • Strip blot of crude rat spinal extract stained with NEFH polyclonal antibody (Cat # PAB14170). A prominent band at an apparent SDS-PAGE molecular weight of 200 KDa corresponds to phosphorylated NF-H.
  • Immunohistochemistry
  • Immunofluorescence
  • Immunofluorescence
  • A section of rat spinal cord stained with NEFH polyclonal antibody (Cat # PAB14170) (green) and ubiquitin C-terminal hydrolase 1 monoclonal antibody (UCHL1) clone MCA-BH7 (red).
    The NEFH polyclonal antibody binds primarily to phosphorylated axonal forms of NEFH, and so stains axons coursing between the large UCHL1 positive neurons.
    These large cells are a-motorneurons and UCHL1 protein is a major component of the perikarya and dendrites of these cells.
  • ELISA
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Immunohistochemistry
  • ELISA
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