Strip blot of crude rat spinal extract stained with NEFH polyclonal antibody (Cat # PAB14170). A prominent band at an apparent SDS-PAGE molecular weight of 200 KDa corresponds to phosphorylated NF-H.
A section of rat spinal cord stained with NEFH polyclonal antibody (Cat # PAB14170) (green) and ubiquitin C-terminal hydrolase 1 monoclonal antibody (UCHL1) clone MCA-BH7 (red). The NEFH polyclonal antibody binds primarily to phosphorylated axonal forms of NEFH, and so stains axons coursing between the large UCHL1 positive neurons. These large cells are a-motorneurons and UCHL1 protein is a major component of the perikarya and dendrites of these cells.