Western blot of HL-60 cell extracts stained with CORO1A polyclonal antibody (Cat # PAB14169), at dilution of 1 : 10,000. A prominent band running with an apparent SDS-PAGE molecular weight of ~ 57 KDa corresponds to CORO1A. Numbers represent positions of molecular weight standards in KDa.
Immunocytochemistry of a mixed neuron/glial culture from newborn rat brain stained with CORO1A polyclonal antibody (Cat # PAB14169) (green) at a dilution of 1 : 10,000 and Chicken Vimentin polyclonal antibody (1 : 5,000). Blue is nuclear DNA counter stain. Glial cells and fibroblasts stain with Vimentin, while microglia alone stain strongly and specifically for CORO1A, which can therefore be used as a robust marker of this important cell type.