Ina polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot of whole rat spinal cord homogenate stained with Ina polyclonal antibody (Cat # PAB14168), at dilution of 1 : 20,000.
A prominent band running at ~ 66 KDa is apparent, as well as smaller lower bands which are apparently degradation products.
A minor band at ~ 150 KDa is also seen, apparently resulting from dimerization of alpha-internexin.
Immunofluorescence
Mixed neuron-glial cultures stained with Ina polyclonal antibody (Cat # PAB14168) (red) and Chicken Peripherin antibody (green).
The Ina antibody stains numerous axonal and dendritic profiles in these cultures, while peripherin antibody binds to only a subset of neurons.
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant Ina.
Immunogen
Recombinant protein corresponding to rat Ina.
Host
Rabbit
Theoretical MW (kDa)
64-66
Reactivity
Rat
Form
Liquid
Recommend Usage
Western Blot (1:10000)
Immunofluorescence (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In serum (5 mM sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot of whole rat spinal cord homogenate stained with Ina polyclonal antibody (Cat # PAB14168), at dilution of 1 : 20,000.
A prominent band running at ~ 66 KDa is apparent, as well as smaller lower bands which are apparently degradation products.
A minor band at ~ 150 KDa is also seen, apparently resulting from dimerization of alpha-internexin.Immunohistochemistry
Immunofluorescence
Mixed neuron-glial cultures stained with Ina polyclonal antibody (Cat # PAB14168) (red) and Chicken Peripherin antibody (green).
The Ina antibody stains numerous axonal and dendritic profiles in these cultures, while peripherin antibody binds to only a subset of neurons.Enzyme-linked Immunoabsorbent Assay
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Gene Info — Ina
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Publication Reference
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Axonally transported peripheral signals regulate alpha-internexin expression in regenerating motoneurons.
McGraw TS, Mickle JP, Shaw G, Streit WJ.
The Journal of Neuroscience 2002 Jun; 22(12):4955.
Application：IHC-P, WB-Ce, Rat, Facial nerve.
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Characterization of mitotic neurons derived from adult rat hypothalamus and brain stem.
Evans J, Sumners C, Moore J, Huentelman MJ, Deng J, Gelband CH, Shaw G.
Journal of Neurophysiology 2002 Feb; 87(2):1076.
Application：IF, WB-Ti, Human, Dog, Rat, Human temporal cortex, Cat frontal cortex, Rat brain stem.
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alpha-Internexin, a 66-kD intermediate filament-binding protein from mammalian central nervous tissues.
Pachter JS, Liem RK.
The Journal of Cell Biology 1985 Oct; 101(4):1316.
Application：WB-Re, Purified proteins.
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Axonally transported peripheral signals regulate alpha-internexin expression in regenerating motoneurons.
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