MARCKS polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
Western blot of whole rat cortex (Lane 1), cerebellum (Lane 2), brain stem (Lane 3) and spinal cord (Lane 4) homogenate stained with MARCKS polyclonal antibody (Cat # PAB14167), at dilution of 1 : 10,000.
A prominent band running with an apparent SDS-PAGE molecular weight of ~ 80 KDa corresponds to MARCKS.
Immunofluorescence
View of mixed neuron/glial cultures stained with MARCKS polyclonal antibody (Cat # PAB14167) (red) and Chicken MAP2 polyclonal antibody.
Note that the MARCKS antibody stains vesicular structures both in the glial cells and in the dendrites of the neurons, which are strongly stained with the MAP2 antibody.
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant MARCKS.
Immunogen
Recombinant protein corresponding to human MARCKS.
Host
Rabbit
Theoretical MW (kDa)
80
Reactivity
Human, Mammals
Form
Liquid
Recommend Usage
Western Blot (1:10000)
Immunofluorescence (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In serum (5 mM sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot of whole rat cortex (Lane 1), cerebellum (Lane 2), brain stem (Lane 3) and spinal cord (Lane 4) homogenate stained with MARCKS polyclonal antibody (Cat # PAB14167), at dilution of 1 : 10,000.
A prominent band running with an apparent SDS-PAGE molecular weight of ~ 80 KDa corresponds to MARCKS.Immunohistochemistry
Immunofluorescence
View of mixed neuron/glial cultures stained with MARCKS polyclonal antibody (Cat # PAB14167) (red) and Chicken MAP2 polyclonal antibody.
Note that the MARCKS antibody stains vesicular structures both in the glial cells and in the dendrites of the neurons, which are strongly stained with the MAP2 antibody.Enzyme-linked Immunoabsorbent Assay
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Gene Info — MARCKS
Entrez GeneID
4082Protein Accession#
DQ341274Gene Name
MARCKS
Gene Alias
80K-L, FLJ14368, FLJ90045, MACS, PKCSL, PRKCSL
Gene Description
myristoylated alanine-rich protein kinase C substrate
Omim ID
177061Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a substrate for protein kinase C. It is localized to the plasma membrane and is an actin filament crosslinking protein. Phosphorylation by protein kinase C or binding to calcium-calmodulin inhibits its association with actin and with the plasma membrane, leading to its presence in the cytoplasm. The protein is thought to be involved in cell motility, phagocytosis, membrane trafficking and mitogenesis. [provided by RefSeq
Other Designations
OTTHUMP00000017045|myristoylated alanine-rich protein kinase C substrate (MARCKS, 80K-L)|phosphomyristin
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Interactome
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Pathway
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Publication Reference
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MARCKS deficiency in mice leads to abnormal brain development and perinatal death.
Stumpo DJ, Bock CB, Tuttle JS, Blackshear PJ.
PNAS 1995 Feb; 92(4):944.
Application：WB-Ti, Mouse, Mouse brains.
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The MARCKS family of cellular protein kinase C substrates.
Blackshear PJ.
The Journal of Biological Chemistry 1993 Jan; 268(3):1501.
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MARCKS is an actin filament crosslinking protein regulated by protein kinase C and calcium-calmodulin.
Hartwig JH, Thelen M, Rosen A, Janmey PA, Nairn AC, Aderem A.
Nature 1992 Apr; 356(6370):618.
Application：WB-Ti, Bovine, Brain.
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MARCKS deficiency in mice leads to abnormal brain development and perinatal death.
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