Western blot of whole rat cortex (Lane 1), cerebellum (Lane 2), brain stem (Lane 3) and spinal cord (Lane 4) homogenate stained with MARCKS polyclonal antibody (Cat # PAB14167), at dilution of 1 : 10,000. A prominent band running with an apparent SDS-PAGE molecular weight of ~ 80 KDa corresponds to MARCKS.
View of mixed neuron/glial cultures stained with MARCKS polyclonal antibody (Cat # PAB14167) (red) and Chicken MAP2 polyclonal antibody. Note that the MARCKS antibody stains vesicular structures both in the glial cells and in the dendrites of the neurons, which are strongly stained with the MAP2 antibody.
The protein encoded by this gene is a substrate for protein kinase C. It is localized to the plasma membrane and is an actin filament crosslinking protein. Phosphorylation by protein kinase C or binding to calcium-calmodulin inhibits its association with actin and with the plasma membrane, leading to its presence in the cytoplasm. The protein is thought to be involved in cell motility, phagocytosis, membrane trafficking and mitogenesis. [provided by RefSeq
OTTHUMP00000017045,myristoylated alanine-rich protein kinase C substrate (MARCKS, 80K-L),phosphomyristin