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Last updated: 2017/2/19
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PPP2R4 polyclonal antibody

  • Catalog # : PAB14148
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of PPP2R4.
  • Immunogen:
  • A synthetic peptide corresponding to human PPP2R4.
  • Sequence:
  • C-GSLLPIHPVTSG
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 40.7, 36.8, 31.9
  • Reactivity:
  • Human, Rat
  • Specificity:
  • Approx 37 KDa band observed in lyastes of human liver tissue and HepG2 cell line (calculated MW of 36.8 KDa according to NP_066954.2).
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Recommend Usage:
  • ELISA (1:32000)
    Western Blot (0.5-2 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • PPP2R4 polyclonal antibody (Cat # PAB14148) (0.5 ug/mL) staining of human liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • PPP2R4 polyclonal antibody (Cat # PAB14148) (0.5 ug/mL) staining of human liver lysate (lane 1), mouse liver (lane 2) and rat liver (lane 3) (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • ELISA
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 5524
  • Protein Accession#:
  • NP_821068.1;NP_066954.2;NP_821070.1
  • Gene Name:
  • PPP2R4
  • Gene Alias:
  • MGC2184,PP2A,PR53,PTPA
  • Gene Description:
  • protein phosphatase 2A activator, regulatory subunit 4
  • Gene Summary:
  • Protein phosphatase 2A is one of the four major Ser/Thr phosphatases and is implicated in the negative control of cell growth and division. Protein phosphatase 2A holoenzymes are heterotrimeric proteins composed of a structural subunit A, a catalytic subunit C, and a regulatory subunit B. The regulatory subunit is encoded by a diverse set of genes that have been grouped into the B/PR55, B'/PR61, and B''/PR72 families. These different regulatory subunits confer distinct enzymatic specificities and intracellular localizations to the holozenzyme. The product of this gene belongs to the B' family. This gene encodes a specific phosphotyrosyl phosphatase activator of the dimeric form of protein phosphatase 2A. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000022333,PP2A phosphatase activator,PP2A, subunit B',phosphotyrosyl phosphatase activator,protein phosphatase 2A, regulatory subunit B',protein phosphatase 2A, regulatory subunit B' (PR 53)
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