PPP2R4 polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
PPP2R4 polyclonal antibody (Cat # PAB14148) (0.5 ug/mL) staining of human liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot (Tissue lysate)
PPP2R4 polyclonal antibody (Cat # PAB14148) (0.5 ug/mL) staining of human liver lysate (lane 1), mouse liver (lane 2) and rat liver (lane 3) (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of PPP2R4.
Immunogen
A synthetic peptide corresponding to human PPP2R4.
Sequence
C-GSLLPIHPVTSG
Host
Goat
Theoretical MW (kDa)
40.7, 36.8, 31.9
Reactivity
Human, Rat
Specificity
Approx 37 KDa band observed in lyastes of human liver tissue and HepG2 cell line (calculated MW of 36.8 KDa according to NP_066954.2).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:32000)
Western Blot (0.5-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
PPP2R4 polyclonal antibody (Cat # PAB14148) (0.5 ug/mL) staining of human liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Western Blot (Tissue lysate)
PPP2R4 polyclonal antibody (Cat # PAB14148) (0.5 ug/mL) staining of human liver lysate (lane 1), mouse liver (lane 2) and rat liver (lane 3) (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — PPP2R4
Entrez GeneID
5524Protein Accession#
NP_821068.1;NP_066954.2;NP_821070.1Gene Name
PPP2R4
Gene Alias
MGC2184, PP2A, PR53, PTPA
Gene Description
protein phosphatase 2A activator, regulatory subunit 4
Omim ID
600756Gene Ontology
HyperlinkGene Summary
Protein phosphatase 2A is one of the four major Ser/Thr phosphatases and is implicated in the negative control of cell growth and division. Protein phosphatase 2A holoenzymes are heterotrimeric proteins composed of a structural subunit A, a catalytic subunit C, and a regulatory subunit B. The regulatory subunit is encoded by a diverse set of genes that have been grouped into the B/PR55, B'/PR61, and B''/PR72 families. These different regulatory subunits confer distinct enzymatic specificities and intracellular localizations to the holozenzyme. The product of this gene belongs to the B' family. This gene encodes a specific phosphotyrosyl phosphatase activator of the dimeric form of protein phosphatase 2A. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq
Other Designations
OTTHUMP00000022333|PP2A phosphatase activator|PP2A, subunit B'|phosphotyrosyl phosphatase activator|protein phosphatase 2A, regulatory subunit B'|protein phosphatase 2A, regulatory subunit B' (PR 53)
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Interactome
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Disease
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Publication Reference
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Protein phosphatase 2A negatively regulates integrin alpha(IIb)beta(3) signaling.
Gushiken FC, Patel V, Liu Y, Pradhan S, Bergeron AL, Peng Y, Vijayan KV.
The Journal of Biological Chemistry 2008 May; 283(19):12862.
Application：IP, WB, Human, HEK 293 cells, platelets.
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Protein phosphatase 2A negatively regulates integrin alpha(IIb)beta(3) signaling.
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